Mouse/Rat PDGF BB ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG83355
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG83355-01 | Antibody Coated Microplate | 8 X 12 strips | 4°C |
| ARG83355-02 | Standard | 3 vials | 4°C |
| ARG83355-03 | 100X Antibody Conjugate | 120 μl | ≤ -20°C |
| ARG83355-04 | 40X Streptavidin-HRP concentrate | 300 μl | 4°C |
| ARG83355-05 | Standard/Sample Diluent buffer | 30 ml | 4°C |
| ARG83355-06 | Antibody Diluent buffer | 30 ml | 4°C |
| ARG83355-07 | 10X Wash Buffer | 50 ml | 4°C |
| ARG83355-08 | TMB substrate | 12 ml | 4°C (protect from light) |
| ARG83355-09 | STOP solution | 12 ml | 4°C |
| ARG83355-10 | Plate sealer | 4 adhesive strips | Room temperature |
概述
| 产品描述 | ARG83355 Mouse/Rat PDGF BB ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse/Rat PDGF BB in serum, plasma or cell culture supernatants. |
|---|---|
| 反应物种 | Ms, Rat |
| 应用 | ELISA |
| 特异性 | No significant cross-reactivity or interference with Human PDGF R alpha |
| 靶点名称 | PDGF BB |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm |
| 灵敏度 | 15.6 pg/ml |
| 样品类型 | Serum, plasma or cell culture supernatants. |
| 标准范围 | 31.25 - 2000 pg/ml |
| 样本量 | 50 µl |
| 精确 | CV: less than10% |
| 別名 | SIS; Becaplermin; Platelet-derived growth factor subunit B; IBGC5; PDGF subunit B; c-sis; SSV; PDGF-2; Proto-oncogene c-Sis; Platelet-derived growth factor beta polypeptide; PDGF2; Platelet-derived growth factor B chain |
应用说明
| 检测时间 | 4 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # P31240 Mouse Platelet-derived growth factor subunit B Swiss-port # Q05028 Rat Platelet-derived growth factor subunit B |
|---|---|
| 基因名称 | PDGFB |
| 全名 | platelet-derived growth factor beta polypeptide |
| 背景介绍 | The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 17, at sites where this gene and that for collagen type 1, alpha 1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2013] |
| 生物功能 | Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA (By similarity). [UniProt] |
| 产品亮点 | Related products: PDGF BB antibodies; PDGF BB ELISA Kits; Related news: The role of HDGF in tumor angiogenesis New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1)
ARG83355 Mouse/Rat PDGF BB ELISA Kit standard curve image
ARG83355 Mouse/Rat PDGF BB ELISA Kitresults of a typical standard run with optical density reading at 450 nm.
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