Mouse sCD40L ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG82083
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82083-001 | Antibody-coated microplate | 8 X 12 strips | 4°C |
| ARG82083-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82083-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82083-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82083-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82083-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C (Protect from light) |
| ARG82083-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82083-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82083-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82083-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82083-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82083 Mouse sCD40L ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse sCD40L in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | sCD40L |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 31.25 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| 标准范围 | 62.5 - 4000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 6.7%; Inter-Assay CV: 7.7% |
| 別名 | TNFSF5; IMD3; T-cell antigen Gp39; HIGM1; CD40-L; gp39; CD40 ligand; Tumor necrosis factor ligand superfamily member 5; CD40L; CD154; TRAP; CD antigen CD154; hCD40L; IGM; T-BAM; TNF-related activation protein |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CD40LG |
| 全名 | CD40 ligand |
| 背景介绍 | The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008] |
| 生物功能 | Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway. [UniProt] |
| 细胞定位 | Cell membrane; Single-pass type II membrane protein. Cell surface. CD40 ligand, soluble form: Secreted. [UniProt] |
| 产品亮点 | Related products: CD40L antibodies; CD40L ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | The soluble form derives from the membrane form by proteolytic processing. N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40. Not O-glycosylated. [UniProt] |
检测图片 (1)
ARG82083 Mouse sCD40L ELISA Kit standard curve image
ARG82083 Mouse sCD40L ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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