Mouse Tissue Factor ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG81904
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81904-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81904-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81904-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81904-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81904-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81904-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81904-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81904-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81904-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81904-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81904-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81904 Mouse Tissue Factor ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse Tissue Factor in serum, plasma (heparin, EDTA, citrate) and cell culture supernatants. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | Tissue Factor |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 7.8 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA, citrate) and cell culture supernatants. |
| 标准范围 | 15.6 - 1000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.7%; Inter-Assay CV: 6.4% |
| 別名 | Thromboplastin; Tissue factor; TFA; CD142; TF; Coagulation factor III; CD antigen CD142 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | F3 |
| 全名 | coagulation factor III (thromboplastin, tissue factor) |
| 背景介绍 | This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010] |
| 生物功能 | Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade. [UniProt] |
| 产品亮点 | Related products: Tissue Factor antibodies; Tissue Factor ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1)
ARG81904 Mouse Tissue Factor ELISA Kit standard curve image
ARG81904 Mouse Tissue Factor ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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