MSH2 Mouse Monoclonal Antibody(ARA725)

Key features and details

Reactivity:Human
Application:WB, IHC
Host:Mouse
Clonality:Monoclonal
Target:MSH2

Brand:

CAT.NO. : ARA6512

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Size:

1mL

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Product Details

Background

MSH2 is a 105 kDa nuclear antigen and encodes a protein of 934 amino acids. The MSH2 antibody gene is one of only 4 known to encode proteins involved in the repair of mismatch nucleotides following DNA replication or repair. Mutations in the MSH2 gene contribute to the development of sporadic colorectal carcinoma. MSHS mutations are responsible for 50% of hereditary non polyposis colorectal cancer (HNPCC). The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related to human carcinogenesis. MSH-2 is involved in the initial cognition of mismatch nucleotides during the replication mismatch repair process. It is thought that after MSH2 binds to a mismatched DNA duplex it is joined by a heterodimer of MLH1 and PMSH, which together help facilitate the later steps in mismatch repair.

Application

Application	Dilution Ratio
WB	1:200-1:5000
IHC-P	1:200-1:1000

Overview

Antibody Type	Recombinant Mouse Monoclonal Antibody
Immunogen	Synthetic peptide within N-terminal residues of Human MSH2.
Species Reactivity	Human, Mouse, Rat
Validated Applications	WB, IHC-P
Molecular Weight	Predicted band size: 105 kDa
Positive Control	HeLa cell lysate, HEK-293 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate, Daudi, human colon cancer tissue, human appendix tissue, human breast cancer tissue, human stomach cancer tissue, rat brain tissue, mouse large intestine tissue.
Conjugation	unconjugated
RRID	AB_3071830
Form	Liquid
Storage Buffer	PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype	IgG1
Purification Method	Protein A affinity purified.