MYC tag Rabbit Monoclonal antibody(ARA678)
Key features and details
- Reactivity:Species independent
- Application:WB, IF/ICC, FC, IP, ELISA
- Host:Rabbit
- Clonality:Monoclonal
- lsotype: IgG1
- Target:MYC tag
-
Brand:
CAT.NO. : ARA6439
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Size:
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Product Details
Background
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The c-Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins.
Application
|
Application |
Dilution Ratio |
|
WB |
1:5000 - 1:40000 |
|
FC |
0.50 ug per 10^6 cells in a 100 µl suspension |
|
IF/ICC |
1:500 - 1:3000 |
|
IP |
0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Overview
|
Applications |
WB, IF/ICC, FC, IP, ELISA |
|
Immunogen |
Peptide |
|
Host / Isotype |
Mouse / IgG1 |
|
Antibody Class |
Monoclonal |
|
Product Type |
Antibody |
|
Full Name |
MYC tag |
|
Alternative Names |
myc, EQKLISEEDL, MYC-tag |
|
RRID |
AB_2734122 |
|
Conjugate Type |
Unconjugated |
|
Form |
Liquid |
|
Purification Method |
Protein G purification |
|
UNIPROT ID |
MYCTAG |
|
Storage Buffer |
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
Data
Western blot of Myc-tagged fusion protein with MYC tag Rabbit Monoclonal antibody(ARA678) at various dilutions.
Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using MYC tag Rabbit Monoclonal antibody(ARA678) at dilution of 1:1000 and Fluor 488-Conjugated AffiniPure Mouse IgG(H+L) Goat Antibody.
IP result of MYC tag Rabbit Monoclonal antibody(ARA678) (IP:4ug; Detection:1:10000) with Transfected HEK-293 cells lysate 300ug.
1x10^6 HEK-293 transfected with GFP-MYC were intracellularly stained with 0.25 ug MYC tag Rabbit Monoclonal antibody(ARA678) and Fluor 647 Mouse IgG(H+L) Goat Antibody . Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.
Storage
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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