Mycoplasma DNA (qPCR) Detection Kit(FAM,VIC）
Key features and details
- Product(cn):支原体DNA（qPCR）检测试剂盒(FAM,CY5)
- Sensitivity:10CFU/mL (10 standard strains)
- Loading Time:70min
- Applicable Instruments:qPCR instruments
- Detection Method:Multiplex PCR
-
Brand:
CAT.NO. : ARK6659
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Size:
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Product Details
Background
Developed independently by AREX, the Mycoplasma DNA (qPCR) Detection Kit is highly specific and sensitive. It enables rapid mycoplasma detection in raw materials, intermediates, and finished products, strictly controlling product quality, avoiding batch scrapping and production delays caused by mycoplasma contamination, and ensuring the safety and effectiveness of biological products.
This kit can be used in conjunction with AREX's "Host Cell Residual DNA (Magnetic Bead Method) Sample Pretreatment Kit" and automatic nucleic acid extractor.
This kit can be used in conjunction with AREX's "Host Cell Residual DNA (Magnetic Bead Method) Sample Pretreatment Kit" and automatic nucleic acid extractor.
Overview
|
Sensitivity |
10CFU/mL (10 standard strains) |
|
Loading Time |
70min |
|
Applicable Instruments |
qPCR instruments |
|
Detection Method |
Multiplex PCR |
|
Fluorescent Dyes |
FAM,VIC |
|
Stability |
Five freeze-thaw cycles do not affect product performance |
Advantage
• Compliance Verification: Validated in accordance with the requirements of multiple national regulations, meeting the application requirements.
• Wide Coverage: Bioinformatics analysis results indicate that it can cover more than 150 mycoplasma sequences.
• Specificity: No cross-reactivity with common sample matrices, genomes of engineered cells/engineered bacteria, and bacteria closely related to mycoplasma parents.
• Comparability: Results are comparable to those of the traditional culture method.
• Wide Coverage: Bioinformatics analysis results indicate that it can cover more than 150 mycoplasma sequences.
• Specificity: No cross-reactivity with common sample matrices, genomes of engineered cells/engineered bacteria, and bacteria closely related to mycoplasma parents.
• Comparability: Results are comparable to those of the traditional culture method.
Mycoplasma Detection Methods Comparison
| Detection Method | qPCR Mycoplasma Rapid Test | USP<63> |
|---|---|---|
| Sample Volume | 0.1 ml | 10 ml |
| Detection Cycle | 3h (AREX) | 28 days |
| Sensitivity | 10 CFU/ml | 10 CFU/ml |
| Advantages | Low laboratory requirements, simple detection method, fast, low cost | Gold standard |
| Disadvantages | Amplification targets mycoplasma DNA | Longer detection time |
Storage
The kit should be stored at -20°C or below, with a shelf life of 12 months.
Please note that any unused portions of the kit after opening should still be stored under the specified storage conditions.
Please note that any unused portions of the kit after opening should still be stored under the specified storage conditions.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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