Myosin Heavy Chain Rabbit Monoclonal Antibody(ARA910)

Key features and details

Reactivity:Human, Mouse, Rat
Application:IHC
Host:Rabbit
Clonality:Monoclonal
Target:Myosin Heavy Chain

Brand:

CAT.NO. : ARA6745

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Size:

1mL

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Product Details

Background

Myosin is a highly conserved, ubiquitously expressed protein that interacts with Actin to generate the force for cellular movements. Conventional myosins are hexameric proteins consisting of two heavy chain subunits, a pair of non-phosphorylatable light chain subunits and a pair of phosphorylatable light chain subunits. Three general classes of myosin have been cloned: smooth muscle myosins, striated muscle myosins and non-muscle myosins. Contractile activity in smooth muscle is regulated by the calcium/calmodulin-dependent phosphorylation of myosin light chain by myosin light chain kinase. Myosin heavy chains are encoded by the MYH gene family and have Actin-activated ATPase activity which generates the motor function of myosin. Myosin heavy chains, which were initially isolated from a human fetal skeletal muscle, are the major determinant in the speed of contraction of skeletal muscle. Various isoforms of myosin heavy chain are differentially expressed depending on the functional activity of the muscle.

Application

Application	Dilution Ratio
IHC-P	1:100-1:500

Overview

Antibody Type	Recombinant Rabbit monoclonal Antibody
Immunogen	Recombinant protein within Human Myosin heavy chain aa 1220-1420 / 1939
Species Reactivity	Human, Mouse, Rat
Validated Applications	WB, IHC-P, IF-Tissue
Molecular Weight	Predicted band size: 224 kDa
Positive Control	Rat skeletal muscle tissue lysates, human skeletal muscle tissue lysates, human skeletal muscle tissue, mouse skeletal muscle tissue, mouse heart tissue, rat skeletal muscle tissue, rat heart tissue, mouse smooth muscle tissue, human striated muscle tissue, human heart tissue
Conjugation	unconjugated
Form	Liquid
Concentration	1μg/μl
Storage Buffer	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide
Isotype	IgG
Purification Method	Protein A affinity purified