Nestin Rabbit Monoclonal Antibody(ARB503)
CAT.NO. : ARB6795
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Background
Nestin is an intermediate filament family member protein that is structurally related to the neurofilament proteins. It is highly expressed in the developing brain, where it may help to regulate cell structure and intracellular processes required for neural cell division and migration.
Research studies have shown that expression of nestin is also found in cells from various nervous system tumors, including gliomas, neuroblastomas, astrocytomas, and it is generally accepted as a marker for neural cancer stem cells.
Nestin expression is significantly increased in melanoma and correlated with more advanced stages of the disease.
Research studies have shown that expression of nestin is also found in cells from various nervous system tumors, including gliomas, neuroblastomas, astrocytomas, and it is generally accepted as a marker for neural cancer stem cells.
Nestin expression is significantly increased in melanoma and correlated with more advanced stages of the disease.
Application
Application |
Dilution Ratio |
IHC |
1:100 - 1:200 |
Overview
Predicted Molecular Wt |
177kDa |
Species Cross-reactivity |
Human |
Applications |
IHC-P |
Purity |
ProA affinity purified IgG |
Form |
Liquid |
Swissprot ID |
P48681 |
Subcellular location |
Cytoplasm |
Recommended Method |
Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18℃-25℃) for 30 minutes |
Storage Buffer |
PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05% |
Storage Conditions |
-25°C to -18°C |
Data

Immunohistochemical staining of human malignant melanoma tissue using Nestin Rabbit Monoclonal Antibody(ARB503)
Storage
Store at -20°C. Stable for one year from the date of shipment.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
