Nestin Rabbit Monoclonal Antibody(ARB503)

Key features and details

  • Target: Nestin
  • Clone ID: ARB503
  • Host: Rabbit
  • Molecular Weight: 177 kDa
  • Purity: ProA affinity purified IgG
  • Species Cross-reactivity: Human
  • Applications: IHC-P
  • Swissprot ID: P48681
  • Brand:
CAT.NO. : ARB6795
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Size:
1mL
Trail, Bulk size or Custom requests Please contact us
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Product Details
Background
Nestin is an intermediate filament family member protein that is structurally related to the neurofilament proteins. It is highly expressed in the developing brain, where it may help to regulate cell structure and intracellular processes required for neural cell division and migration.
Research studies have shown that expression of nestin is also found in cells from various nervous system tumors, including gliomas, neuroblastomas, astrocytomas, and it is generally accepted as a marker for neural cancer stem cells.
Nestin expression is significantly increased in melanoma and correlated with more advanced stages of the disease.
Application

Application

Dilution Ratio

IHC

1:100 - 1:200

Overview

Predicted Molecular Wt

177kDa

Species Cross-reactivity

Human

Applications

IHC-P

Purity

ProA affinity purified IgG

Form

Liquid

Swissprot ID

P48681

Subcellular location

Cytoplasm

Recommended Method

Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18℃-25℃) for 30 minutes

Storage Buffer

PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%

Storage Conditions

-25°C to -18°C

Data

Immunohistochemical staining of human malignant melanoma tissue using Nestin Rabbit Monoclonal Antibody(ARB503)
Storage
Store at -20°C. Stable for one year from the date of shipment.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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