Neuron Development Marker Antibody Duo (Nestin, Tuj1)

Key features and details

  • 产品描述: During embryonic neurogenesis, neural progenitors give rise to intermediate progenitors, before they are further differentiated into premature neurons. In the telencephalon, neural progenitor cells express Nestin and losses its expression soon after they committed their fate to becoming neurons. Premature neurons express TujI/TUBB3, which has been commonly used as a marker to identify neural differentiation in the developing brain or even in stem cell studies.arigo grouped Nestin and TujI as control duo to offer reliable antibodies for the distinction of neural progenitors and premature neurons during the development of the central nervous system.Related news:Neuronal Development Marker
  • 靶点名称: Neuron Development Marker
  • 別名: Neuron Development Marker antibody; Nestin antibody; beta III Tubulinantibody
  • Brand:
CAT.NO. : ARG30008
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Product Details
内含物
货号内含物名称宿主克隆性反应应用包装
ARG52345anti-Nestin antibody [4D11]Mouse mAbHu, Ms, RatICC/IF, IHC-P, WB50 μl
ARG65321anti-beta III Tubulin antibodyGoat pAbHuWB50 μg
概述
产品描述During embryonic neurogenesis, neural progenitors give rise to intermediate progenitors, before they are further differentiated into premature neurons. In the telencephalon, neural progenitor cells express Nestin and losses its expression soon after they committed their fate to becoming neurons. Premature neurons express TujI/TUBB3, which has been commonly used as a marker to identify neural differentiation in the developing brain or even in stem cell studies.
arigo grouped Nestin and TujI as control duo to offer reliable antibodies for the distinction of neural progenitors and premature neurons during the development of the central nervous system.

Related news:
Neuronal Development Marker
靶点名称Neuron Development Marker
別名Neuron Development Marker antibody; Nestin antibody; beta III Tubulinantibody
属性
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
全名Antibody Duo for Neuron Development Marker (Nestin, Tuj1)
研究领域Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody
检测图片 (7)
  • ARG52345 anti-Nestin antibody [4D11] ICC/IF image

    Immunofluorescence: Cultured neonatal Rat neurons and glia showing nestin labeling in red (ARG52345 Nestin antibody [4D11]) and vimentin (ARG52468 anti-Vimentin antibody) in green.

    Astrocytes and neuronal stem cells stain strongly and specifically in a clearly filamentous fashion with the anti-Nestin antibody. The presence of Nestin indicates that the cells are developing astrocytes, neuroblasts or undifferentiated neural stem cells.

  • ARG52345 anti-Nestin antibody [4D11] ICC/IF image

    Immunofluorescence: Cortical neuron-glial cells from E20 Rat stained with ARG52345 anti-Nestin antibody [4D11] (red) at 1:500 dilution and costained with ARG52328 anti-MAP2 antibody (green) at 1:5000 dilution. Hoechst (blue) for nuclear staining.

    The Nestin antibody labels developing astrocytes and neuronal stem cells in a clearly filamentous fashion, while the MAP2 antibody stains dendrites and perikarya of mature neurons.

  • ARG52345 anti-Nestin antibody [4D11] WB image

    Western Blot: neonatal rat brain lysate stained with Nestin antibody (ARG52345) showing specific immunolabeling of the ~220-240 kDa nestin doublet.

  • ARG65321 anti-TUBB3 antibody WB image

    Western Blot: HepG2 lysate (35 µg protein in RIPA buffer) stained with ARG65321 anti-Tubulin beta III antibody at 2 µg/ml dilution.

  • ARG52345 anti-Nestin antibody [4D11] WB image

    Western blot: Rat brain (P18) homogenate stained with ARG52345 anti-Nestin antibody [4D11].

  • ARG52345 anti-Nestin antibody [4D11] WB image

    Western blot: Rat brain (embryonic, E18), C6 and SH-SY5Y cell lysates stained with ARG52345 anti-Nestin antibody [4D11] (green) at 1:500 dilution.

  • ARG52345 anti-Nestin antibody [4D11] ICC/IF image

    Immunofluorescence: cultured neonatal rat neurons and glia showing nestin labeling in red (ARG52345 Nestin antibody [4D11]) and vimentin (ARG52468 anti-Vimentin antibody) in green. Astrocytes and neuronal stem cells stain strongly and specifically in a clearly filamentous fashion with the anti-Nestin antibody. The presence of Nestin indicates that the cells are developing astrocytes, neuroblasts or undifferentiated neural stem cells.

参考文献

Electrical stimulation induced pre-vascularization of engineered dental pulp tissue

ARG52345: ICC/IF / Human

Ying-tong Wang et al.
Regenerative Therapy,  (2024)

publication_link

 

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Insulin-like growth factor reduced against decabromodiphenyl ether-209-induced neurodevelopmental toxicity in vivo and in vitro

ARG52345: ICC/IF / Rat

Yuanxiang Yang et al.
Hum Exp Toxicol.,  (2022)

publication_link

 

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Mesenchymal Stem Cell-Derived Exosomes Ameliorate Alzheimer's Disease Pathology and Improve Cognitive Deficits.

ARG52345: WB / Human

Yi-An Chen et al.
Biomedicines.,  (2021)

publication_link

 

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Transplantation of encapsulated human Leydig-like cells: A novel option for the treatment of testosterone deficiency.

ARG52345: ICC/IF / Human

Xin Feng et al.
Mol Cell Endocrinol.,  (2020)

publication_link

 

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