Nitrite Assay Kit

Key features and details

  • 产品描述: ARG83399 Nitrite Assay Kit can be used to measure Nitrite in serum, plasma, urine, tissue extracts, cell lysate, cell culture media and other biological fluids.
  • 反应物种: Other
  • 应用: FuncSt
  • 靶点名称: Nitrite
  • 偶联标记说明: Read at 547 nm
  • 灵敏度: 0.005 nmol/ml
  • 样品类型: Serum, plasma, urine, tissue extracts, cell lysate, cell culture media and other biological fluids.
  • 标准范围: 0.01 nmol/ml - 1 nmol/ml
  • 样本量: 170 μl
  • Brand:
CAT.NO. : ARG83399
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Product Details
概述
产品描述ARG83399 Nitrite Assay Kit can be used to measure Nitrite in serum, plasma, urine, tissue extracts, cell lysate, cell culture media and other biological fluids.
反应物种Other
应用FuncSt
靶点名称Nitrite
偶联标记说明Read at 547 nm
灵敏度0.005 nmol/ml
样品类型Serum, plasma, urine, tissue extracts, cell lysate, cell culture media and other biological fluids.
标准范围0.01 nmol/ml - 1 nmol/ml
样本量170 μl
別名Nitrite; Nitrite ion;Nitrous acid, ion(1-); Nitrite ion(1-); Nitrogen peroxide ion(1-)
应用说明
检测时间20 min
属性
形式96 well
存放说明Store the kit at 4°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
背景介绍The nitrite ion has the chemical formula NO−2. Nitrite (mostly sodium nitrite) is widely used throughout chemical and pharmaceutical industries. The nitrite anion is a pervasive intermediate in the nitrogen cycle in nature. The name nitrite also refers to organic compounds having the –ONO group, which are esters of nitrous acid.
生物功能Nitrite can be reduced to nitric oxide or ammonia by many species of bacteria. Under hypoxic conditions, nitrite may release nitric oxide, which causes potent vasodilation. Several mechanisms for nitrite conversion to NO have been described, including enzymatic reduction by xanthine oxidoreductase, nitrite reductase, and NO synthase (NOS), as well as nonenzymatic acidic disproportionation reactions.
检测图片 (1)
  • ARG83399 Nitrite Assay Kit standard curve image

    ARG83399 Nitrite Assay Kitresults of a typical standard run with optical density reading at 450 nm.

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