Nitrite reductase Assay Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 靶点名称:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
- 标准范围:
- 样本量:
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Brand:
CAT.NO. : ARG83413
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Product Details
概述
| 产品描述 | ARG83413 Nitrite reductase Assay Kit can be used to measure Nitrite reductase in tissue extracts, cell lysate, cell culture media and other biological fluids. |
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| 反应物种 | Other |
| 应用 | FuncSt |
| 靶点名称 | Nitrite reductase |
| 偶联标记说明 | Read at 540 nm |
| 灵敏度 | 0.01 μmol/ml |
| 样品类型 | Tissue extracts, cell lysate, cell culture media and other biological fluids. |
| 标准范围 | 0.02 μmol/ml - 2 μmol/ml |
| 样本量 | 20 μl |
| 別名 | Nitrite reductase |
应用说明
| 检测时间 | 30 min |
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属性
| 形式 | 96 well |
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| 存放说明 | Store the kit at 4°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 背景介绍 | Nitrite reductase (NIR) has been determined to be the major NO producer in mammalian cells under hypoxic conditions, such as those that commonly occur in hypoxic vasodilation of cardiovascular systems, muscle tissue during extensive exercise, and ischemic tissues. Unlike NOS, NIR produces NO in an O2-independent manner, suggesting the potential contribution of NO in mediating hypoxic signalling-dependent cellular responses. Haem-binding proteins, such as haemoglobin and myoglobin, and cytochrome c, cytochrome bc1, and cytochrome c oxidase of the mitochondrial electron transport chain, have been reported to generate NO via NIR-dependent catalysis. |
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