NLRP3 Rabbit Polyclonal Antibody
Key features and details
- Reactivity:Human, Mouse, Rat
- Application:WB, Flow, ICC/IF, IHC
- Host:Rabbit
- Clonality:Polyclonal
- lsotype:IgG
- Target:NLRP3
-
Brand:
CAT.NO. : ARA6619
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Product Details
Background
This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site domain, and a leucine-rich repeat motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome, and neonatal-onset multisystem inflammatory disease. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid.
Application
|
Applications |
Tested Dilution |
|
Western Blot (WB) |
1:500-1:5000 |
|
Immunohistochemistry (IHC) |
1:50-200 |
|
Immunohistochemistry (Paraffin) (IHC (P)) |
1:50-200 |
|
Immunocytochemistry (ICC/IF) |
- |
|
Flow Cytometry (Flow) |
- |
Overview
|
Species Reactivity |
Human, Mouse, Rat |
|
Application |
WB, Flow, ICC/IF, IHC |
|
Host/Isotype |
Rabbit / IgG |
|
Class |
Polyclonal |
|
Conjugate |
Unconjugated |
|
Immunogen |
This NLRP3/NALP3 antibody was raised against a portion of amino acids 1-50 of human NLRP3/NALP3. |
|
Form |
Lyophilized |
|
Concentration |
1mg/mL |
|
Purification |
Antigen affinity chromatography |
|
Storage buffer |
PBS with 4mg trehalose |
|
Contains |
no preservative |
Data
Western Blot: NLRP3/NALP3 Antibody [ARA6619] - Analysis of NALP3 using NALP3 antibody. Lane A) Human NALP3 transfected cell lysate, B) Mouse NALP3 transfected cell lysate, and C) HEK293 control lysate probed with NALP3 antibody at 3 ug/mL.
Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody [ARA6619] - Analysis of human esophagus using NLRP3/NALP3 antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems).
Storage
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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