NSC and Neuron Marker Antibody Duo (Nestin, MAP2)
Key features and details
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- 靶点名称:
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Product Details
| 货号 | 内含物名称 | 宿主克隆性 | 反应 | 应用 | 包装 |
|---|---|---|---|---|---|
| ARG52328 | anti-MAP2 antibody | Chicken pAb | Hu, Ms, Rat, Bov, Dog, Marmoset, Sheep | ICC/IF, IHC-FoFr , IHC-Fr, IHC-P, WB | 50 μl |
| ARG52345 | anti-Nestin antibody [4D11] | Mouse mAb | Hu, Ms, Rat | ICC/IF, IHC-P, WB | 50 μl |
| 产品描述 | Nestin is an intermediate filament protein expressed in dividing cells during the early development stages of nervous system and is utilized as a neural stem cell marker. The neural stem cells loss Nestin expression once the cell fate is determined. Following neuro- and gliogenesis, Nestin is replaced by cell type-specific intermediate filaments, e.g., Neurofilaments for neurons and GFAP for astrocytes. MAP2 is a neuron-specific cytoskeletal protein that enriches and stabilizes in dendrites and is used as a mature neuron marker. arigo’s ARG30009 NSC and Neuron Marker Antibody Duo (Nestin, MAP2) is excellent for distinguishing neural stem cells and mature neurons. Related news: 14-3-3η as a promising target for the treatment of Major Depression Disorder Neuronal Development Marker Astrocyte-to-neuron conversion for Parkinson's disease treatment |
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| 靶点名称 | NSC and Neuron Marker |
| 別名 | NSC and Neuron Marker antibody; MAP2 antibody; Nestin antibody |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
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| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 全名 | Antibody Duo for NSC and Neuron Marker (Nestin, MAP2) |
|---|---|
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody |
ARG52345 anti-Nestin antibody [4D11] ICC/IF image
Immunofluorescence: Cultured neonatal Rat neurons and glia showing nestin labeling in red (ARG52345 Nestin antibody [4D11]) and vimentin (ARG52468 anti-Vimentin antibody) in green.
Astrocytes and neuronal stem cells stain strongly and specifically in a clearly filamentous fashion with the anti-Nestin antibody. The presence of Nestin indicates that the cells are developing astrocytes, neuroblasts or undifferentiated neural stem cells.
ARG52345 anti-Nestin antibody [4D11] ICC/IF image
Immunofluorescence: Cortical neuron-glial cells from E20 Rat stained with ARG52345 anti-Nestin antibody [4D11] (red) at 1:500 dilution and costained with ARG52328 anti-MAP2 antibody (green) at 1:5000 dilution. Hoechst (blue) for nuclear staining.
The Nestin antibody labels developing astrocytes and neuronal stem cells in a clearly filamentous fashion, while the MAP2 antibody stains dendrites and perikarya of mature neurons.
ARG52328 anti-MAP2 antibody WB image
Western Blot: rat cortex lysate stained with ARG52328 anti-MAP2 antibody showing specific immunolabeling of the ~280k MAP2 protein.
ARG52345 anti-Nestin antibody [4D11] WB image
Western Blot: neonatal rat brain lysate stained with Nestin antibody (ARG52345) showing specific immunolabeling of the ~220-240 kDa nestin doublet.
ARG52345 anti-Nestin antibody [4D11] WB image
Western blot: Rat brain (P18) homogenate stained with ARG52345 anti-Nestin antibody [4D11].
ARG52345 anti-Nestin antibody [4D11] WB image
Western blot: Rat brain (embryonic, E18), C6 and SH-SY5Y cell lysates stained with ARG52345 anti-Nestin antibody [4D11] (green) at 1:500 dilution.
ARG52328 anti-MAP2 antibody ICC/IF image
Immunofluorescence: Mixed neuron/glial cultures. The perikarya and dendrites of neurons are strongly and specifically stained with ARG52328 anti-MAP2 antibody (red). Cell nuclei are visualized with DAPI DNA stain.
ARG52345 anti-Nestin antibody [4D11] ICC/IF image
Immunofluorescence: cultured neonatal rat neurons and glia showing nestin labeling in red (ARG52345 Nestin antibody [4D11]) and vimentin (ARG52468 anti-Vimentin antibody) in green. Astrocytes and neuronal stem cells stain strongly and specifically in a clearly filamentous fashion with the anti-Nestin antibody. The presence of Nestin indicates that the cells are developing astrocytes, neuroblasts or undifferentiated neural stem cells.
ARG52328 anti-MAP2 antibody ICC/IF image
Immunofluorescence: E17 Rat midbrain mixed neuronal cultures stained with ARG52461 anti-Tyrosine Hydroxylase antibody (green) and ARG52328 anti-MAP2 antibody (red).
Magnetic stirring with iron oxide nanospinners accretes neurotoxic Aβ42 oligomers into phagocytic clearable plaques for Alzheimer's disease treatment
ARG52328: ICC/IF / Mouse
Electrical stimulation induced pre-vascularization of engineered dental pulp tissue
ARG52345: ICC/IF / Human
Insulin-like growth factor reduced against decabromodiphenyl ether-209-induced neurodevelopmental toxicity in vivo and in vitro
ARG52345: ICC/IF / Rat
Mesenchymal Stem Cell-Derived Exosomes Ameliorate Alzheimer's Disease Pathology and Improve Cognitive Deficits.
ARG52345: WB / Human
Transplantation of encapsulated human Leydig-like cells: A novel option for the treatment of testosterone deficiency.
ARG52345: ICC/IF / Human
Extracellular RNAs-TLR3 signaling contributes to cognitive decline in a mouse model of postoperative cognitive dysfunction.
ARG52328: IHC-Fr / Mouse
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