NSE Mouse Monoclonal Antibody(ARB509)
CAT.NO. : ARB6801
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Background
NSE is an intracytoplasmic protein, and enolase is a dimer in mammals. It has three subunits: 1, 2, and 3, which are mainly distributed in central nerve, peripheral nerve, neuroendocrine cells and tumor cells. This antibody can specifically recognize 47kDa of NSE, and can be used for the auxiliary diagnosis of neuroendocrine tumors. Due to the poor specificity of the antibody, some normal smooth muscle, myoepithelial cells, renal tubular cells, lymphocytes can also express NSE. Therefore, this antibody is not used as a single diagnostic marker, and should be applied in combination with other indicators to improve its diagnostic accuracy.
Application
Application |
Dilution Ratio |
IHC |
1:100 - 1:200 |
Overview
Predicted Molecular Wt |
47kDa |
Species Cross-reactivity |
Human |
Applications |
IHC-P |
Purity |
ProG affinity purified IgG |
Form |
Liquid |
Swissprot ID |
P09104 |
Subcellular location |
Cytoplasm |
Recommended method |
Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18℃-25℃) for 30 minutes |
Immunogen |
Synthetic peptide within Human NSE aa 416-433. |
Storage Buffer |
PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05% |
Storage conditions |
-25°C to -18°C |
Data

Immunohistochemical staining of human pheochromocytoma tissue using NSE Mouse Monoclonal Antibody(ARB509)
Storage
Store at -20°C. Stable for one year from the date of shipment.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
