Oct4 Rabbit Monoclonal Antibody(ARA864)

Key features and details

Reactivity:Human
Application:IHC
Host:Rabbit
Clonality:Monoclonal
Target:Oct4

Brand:

CAT.NO. : ARA6690

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Size:

1mL

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Product Details

Background

POU5F1 (POU domain, class 5, transcription factor 1), also known as octamer - binding transcription factor-3 oct 3, OCT3), octamer - binding transcription factor-4 (Oct-4, Oct4 and Oct-3/4), modulates embryonic stem (ES) cell populations by influencing lineage commitment. Oct-3/4 sustains stem - cell self - renewal and differentiation pathways. Transcription factors containing the POU homeodomain regulate tissue - specific gene expression in lymphoid and pituitary and in early mammalian development. Oct-3/4 is capable of inducing rapid differentiation and tumorigenic properties of ES cells through activation of the UTF1 gene. In humans, two Oct-3/4 isoforms contribute to influencing the undifferentiated phenotype of ES cells. Oct-3/4 pseudogenes localizing to human chromosomes 10 and 8 are reported to be transcribed in certain cancer cell lines and tissues.

Application

Application	Dilution Ratio
IHC-P	1:500-1:4000

Overview

Antibody Type	Recombinant Rabbit monoclonal Antibody
Immunogen	Synthetic peptide within human Oct4 aa 20-60
Species Reactivity	Human, Mouse
Validated Applications	WB, IP-Cell, IF-Tissue, IHC-P, IP, FC, ChIP
Molecular Weight	Predicted band size: 39 kDa
Positive Control	F9 cell lysate, NCCIT cell lysate, NCCIT, F9, mouse liver tissue, human seminoma tissue tissue
Conjugation	unconjugated
RRID	AB_3070093
Form	Liquid
Concentration	1μg/ul
Storage Buffer	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol, Preservative: 0.05% Sodium Azide
Isotype	IgG
Purification Method	Protein A affinity purified