P-S6 Rabbit Monoclonal Antibody(ARA891)

Key features and details

Reactivity:Human, Mouse, Rat
Application:IHC
Host:Rabbit
Clonality:Monoclonal
Target:P-S6

Brand:

CAT.NO. : ARA6718

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Size:

1mL

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Product Details

Background

This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17.

Application

Application	Dilution Ratio
IHC-P	1:100-1:1000

Overview

Antibody Type	Recombinant Rabbit monoclonal Antibody
Immunogen	Synthetic peptide within human P70 S6 Kinase aa 371-420 / 525
Species Reactivity	Human, Mouse, Rat
Validated Applications	WB, IP-Cell, IHC-P
Molecular Weight	Predicted band size: 59 kDa
Positive Control	MCF7 cell lysate, MCF7 treated with 20% FBS overnight then add 100nM Calyculin A for 30 minutes cell lysate, NIH/3T3 treated with 20% FBS overnight then add 100nM Calyculin A for 30 minutes cell lysate, human breast carcinoma tissue, MCF7 starved for 4 hours then treated with 200ng/mL EGF for 15 minutes cell lysate, C6 cell lysate, C6 treated with 100nM Calyculin A for 30 minutes cell lysate, human liver carcinoma tissue, 293T cell lysate, 293T serum starved overnight cell lysate, 293T serum starved overnight then treated with 100nM Insulin for 15 minutes cell lysate, MCF7 cells treated with 20% FBS overnight then add 100nM Calyculin A for 30 minutes
Conjugation	unconjugated
RRID	AB_3072915
Form	Liquid
Concentration	1μg/ul
Storage Buffer	PBS (pH7.4), 0.1% BSA, 40% Glycerol, Preservative: 0.05% Sodium Azide
Isotype	IgG
Purification Method	Protein A affinity purified