Pan-Lactyl-lysine Rabbit Monoclonal Antibody(ARA826)
Key features and details
- Reactivity:Species independent
- Application:WB, IHC-P, IF-Cell, FC, Dot Blot
- Host:Rabbit
- Clonality:Monoclonal
- lsotype:IgG
- Target:Pan-Lactyl-lysine
-
Brand:
CAT.NO. : ARA6626
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Size:
Trail, Bulk size or Custom requests Please contact us
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Product Details
Background
Post-translational modifications (PTMs) represent a crucial means of regulating diverse biological processes and cellular physiology by influencing protein structure and function. Histone lysine lactylation (Kla) is a newly discovered histone modification, which regulates gene expression in macrophages. In M1 macrophages, lactate is derived from incompletely oxidized glucose and then generate lactyl-CoA, which is transferred to lysine tails of histone proteins via the acetyl transferase p300. This modification was high in gene promoter regions that lack acetylation and associated with activation of genes expression. As a large number of studies have demonstrated that lysine acylation possessed a diverse range of substrate proteins, however, no systematic analysis has been reported for lysine lactylation.
Application
|
Application |
Dilution Ratio |
|
WB |
1:2,000 |
|
IHC-P |
1:500 - 1:1,000 |
|
IF-Cell |
1:1,000 |
|
FC |
1:1,000 |
|
Dot Blot |
1:2,000 |
Overview
|
Antibody Type |
Recombinant Rabbit monoclonal Antibody |
|
Immunogen |
Synthetic Lactyl lysine-containing peptide. |
|
Species Reactivity |
Species independent |
|
Validated Applications |
WB, IHC-P, IF-Cell, FC, Dot Blot |
|
Positive Controls |
HeLa treated with 100mM Lactate sodium for 24 hours cell lysate, human breast cancer tissue, human colon cancer tissue, mouse small intestine tissue, rat small intestine tissue, HeLa cells treated with 100mM Lactate sodium for 24 hours. |
|
Conjugation |
unconjugated |
|
RRID |
AB_3096499 |
|
Form |
Liquid |
|
Concentration |
1μg/μl |
|
Storage Buffer |
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
|
Isotype |
IgG |
|
Purification Method |
Protein A affinity purified. |
Data
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Pan-Lactyl-lysine antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-Pan-Lactyl-lysine antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue with Rabbit anti-Pan-Lactyl-lysine antibody at 1/500 dilution.
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Rabbit anti-Pan-Lactyl-lysine antibody at 1/500 dilution.
Storage
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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