PCB Rabbit Monoclonal Antibody(ARA718)

Key features and details

Reactivity:Human, Mouse, Rat
Application:WB, IHC, IF/ICC, FC, IP
Host:Rabbit
Clonality:Monoclonal
lsotype: IgG
Target:PCB

Brand:

CAT.NO. : ARA6493

RMB Please choose

Size:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

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Product Details

Background

Pyruvate carboxylase (PC) encoded by the gene PC is an enzyme (EC 6.4.1.1) of the ligase class that catalyzes (depending on the species) the physiologically irreversible carboxylation of pyruvate to form oxaloacetate (OAA). It is an important anaplerotic reaction that creates oxaloacetate from pyruvate. PC contains a biotin prosthetic group and is typically localized to the mitochondria in eukaryotes with exceptions to some fungal species such as Aspergillus nidulans which have a cytosolic PC. PC requires magnesium and zinc or manganese for catalysis. PC from different organisms exhibit varying degrees of activation by acetyl-CoA, but vertebrate PC typically requires it for activity.

Application

Application	Dilution Ratio
WB	1:1,000 - 1:5,000
IHC	1:180 - 1:200
IF	1:200 - 1:250
FC	1:800 - 1:1,000
IP	1-2μg/sample

Overview

Antibody Type	Recombinant Rabbit monoclonal Antibody
Immunogen	Recombinant protein within Human PCB aa 450-1178.
Species Reactivity	Human, Mouse, Rat
Validated Applications	WB, IHC, IF/ICC, FC, IP
Molecular Weight	Predicted band size: 130 kDa
Positive Control	HepG2 cell lysate, MCF7 cell lysate, A549 cell lysate, Huh7 cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Mouse kidney tissue lysate, Mouse liver tissue lysate, Mouse lymph node tissue lysate, Rat liver tissue lysate, Rat brain tissue lysate, HepG2, PC-12, mouse liver tissue, mouse lymph node tissue, rat liver tissue, rat brain tissue.
Conjugation	unconjugated
RRID	AB_3072811
Form	Liquid
Storage Buffer	PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype	IgG
Purification Method	Protein A affinity purified.

Data

Western blot analysis of PCB on different lysates with PCB Rabbit Monoclonal Antibody(ARA718).

Immunocytochemistry analysis of HepG2 cells labeling PCB with PCB Rabbit Monoclonal Antibody(ARA718).

Immunohistochemical analysis of paraffin-embedded rat liver tissue with PCB Rabbit Monoclonal Antibody(ARA718).

Flow cytometric analysis of PC-12 cells labeling PCB.

PCB was immunoprecipitated in 0.2mg HeLa cell lysate with PCB Rabbit Monoclonal Antibody(ARA718) at 2 µg/10 µl beads.

Storage

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Research Use Only

For Research Use Only. Not for use in diagnostic procedures.

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