Phosphatidylcholine Assay Kit (Fluorometric)
CAT.NO. : ARG82860
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概述
产品描述 | ARG82860 Phosphatidylcholine Assay Kit (Fluorometric) can be used to measure Phosphatidylcholine levels in plasma or serum, tissue homogenates, or cell culture supernatants. |
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反应物种 | Other |
应用 | FuncSt |
靶点名称 | Phosphatidylcholine |
偶联标记说明 | λ ex/em = 530-570 / 590-600 nm (fluorometric) |
灵敏度 | 0.5 mg/dL |
样品类型 | Plasma or serum, tissue homogenates, or cell culture supernatants. |
标准范围 | 0.156 - 10 mg/dL |
样本量 | 10 µl |
应用说明
检测时间 | ~60 -70 minutes |
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属性
形式 | 96 well |
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存放说明 | Store components at 4°C, -20°C or -80°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
背景介绍 | Phosphatidylcholines (PC) are a class of phospholipids that incorporate choline as a headgroup. They are a major component of biological membranes and can be easily obtained from a variety of readily available sources, such as egg yolk or soybeans, from which they are mechanically or chemically extracted using hexane. They are also a member of the lecithin group of yellow-brownish fatty substances occurring in animal and plant tissues. Dipalmitoyl phosphatidylcholine (a.k.a. lecithin) is a major component of pulmonary surfactant and is often used in the L/S ratio to calculate fetal lung maturity. While phosphatidylcholines are found in all plant and animal cells, they are absent in the membranes of most bacteria, including Escherichia coli. Purified phosphatidylcholine is produced commercially. Phospholipase D catalyzes the hydrolysis of phosphatidylcholine to form phosphatidic acid (PA), releasing the soluble choline headgroup into the cytosol. [Wikipedia: Phosphatidylcholine] |
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