Phospho-Erk1 (T202 + Y204) + Erk2 (T185 + Y187) Rabbit Monoclonal Antibody(ARA723)

Key features and details

Reactivity:Human, Mouse, Rat
Application:WB, IHC, IF/ICC, FC
Host:Rabbit
Clonality:Monoclonal
lsotype: IgG
Target:Phospho-Erk1 (T202 + Y204) + Erk2 (T185 + Y187)

Brand:

CAT.NO. : ARA6509

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Size:

50μL 100μL

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Product Details

Background

The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at Tyrosine 204 and 187 and Threonine 177 and 160 residues mapping within a characteristic Thr-Glu-Tyr motif. Phosphorylation at both the Threonine 202 and Tyrosine 204 residues of ERK1 and Threonine 185 and Tyrosine 187 residues of ERK2 is required for full enzymatic activation. The structural consequences of dual-phosphorylation in the ERK2 include active site closure, alignment of key catalytic residues that interact with ATP, and remodeling of the activation loop. In response to activation, MAP kinases phosphorylate downstream components on serine and threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6.

Application

Application	Dilution Ratio
WB	1:5,000 - 1:10,000
IF/ICC	1:80 - 1:100
IHC	1:800 - 1:1,000
FC	1:800 - 1:1,000

Overview

Antibody Type	Recombinant Rabbit monoclonal Antibody
Immunogen	Synthetic peptide within Human ERK1 aa 166-215 / 379.
Species Reactivity	Human, Mouse, Rat
Validated Applications	WB, IHC, IF/ICC, FC
Molecular Weight	Predicted band size: 41/43 kDa
Positive Control	SH-SY5Y cell lysate, SH-SY5Y treated with 100ng/mL hβ-NGF for 10 minutes cell lysate, PC-12 treated with 100ng/mL hβ-NGF for 10 minutes cell lysate, SK-Br-3 whole cell lysate, NIH/3T3 cell lysate, NIH/3T3 treated with 200nM PMA for 30 minutes cell lysate, HeLa treated with 200nM PMA for 30 minutes cell lysate, human thyroid carcinoma tissue.
Conjugation	unconjugated
RRID	AB_3069896
Form	Liquid
Storage Buffer	1×TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype	IgG
Purification Method	Protein A affinity purified.

Data

Western blot analysis of Phospho-Erk1 (T202 + Y204) + Erk2 (T185 + Y187) on different lysates with Phospho-Erk1 (T202 + Y204) + Erk2 (T185 + Y187) Rabbit Monoclonal Antibody(ARA723).

Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue with Phospho-Erk1 (T202 + Y204) + Erk2 (T185 + Y187) Rabbit Monoclonal Antibody(ARA723).

Immunocytochemistry analysis of NIH/3T3 cells treated with 200nM PMA for 30 minutes labeling Phospho-Erk1 (T202 + Y204) + Erk2 (T185 + Y187) with Phospho-Erk1 (T202 + Y204) + Erk2 (T185 + Y187) Rabbit Monoclonal Antibody(ARA723).

Flow cytometric analysis of HeLa cells untreated (left) or treated (right) with PMA for 30 minutes labeling Phospho-Erk1 (T202 + Y204) + Erk2 (T185 + Y187).

Storage

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Research Use Only

For Research Use Only. Not for use in diagnostic procedures.

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