Phospho NFkB p65 Antibody Duo (Total, pT435)

产品描述: Nuclear factor kappa B (NF-κB) is a protein complex that controls large number of genes responsible for the regulation of apoptosis, inﬂammation, tumorigenesis and a variety of immune responses. NF-kB is sensitive and responsive to stimuli such as cytokines, stress, UV, low oxygen, bacterial or viral infections. In most cell types, NF-κB subunits exist as hetero- or homodimers bound to the inhibitory IκB proteins, which retain the complex mainly in the cytoplasm. Once activated, IκB proteins are phosphorylated by IKKα and IKKβ, leading to the degradation of IκB proteins and nuclear translocation of NF-κB proteins. Post-translation modification such as phosphorylation of NF-κB p65 at Thr435 site leads to transcription activity and NF-κB subunit transactivation. O’Shea and Perkins. Biochem J. 2010. 426: 345-354Hayden and Ghosh. (2012) Genes and Dev 26: 203-234 Baeuerle and Henkel. (1994) Ann Rev Immunol 12: 141-179Related news:Exploring Antiviral Immune Response
靶点名称: NFkB p65
別名: Phospho NFkB p65 antibody; NFkB p65 phospho (Thr435) antibody; NFkB p65 antibody

CAT.NO. : ARG30254

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Size:

1 pair

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Product Details

内含物

货号	内含物名称	宿主克隆性	反应	应用	包装
ARG51516	anti-NFkB p65 phospho (Thr435) antibody	Rabbit pAb	Hu, Ms, Rat	IHC-P, WB	50 μl
ARG65677	anti-NFkB p65 antibody	Mouse mAb	Hu, Ms, Rat	IHC-P, WB	50 μg

概述

产品描述	Nuclear factor kappa B (NF-κB) is a protein complex that controls large number of genes responsible for the regulation of apoptosis, inﬂammation, tumorigenesis and a variety of immune responses. NF-kB is sensitive and responsive to stimuli such as cytokines, stress, UV, low oxygen, bacterial or viral infections. In most cell types, NF-κB subunits exist as hetero- or homodimers bound to the inhibitory IκB proteins, which retain the complex mainly in the cytoplasm. Once activated, IκB proteins are phosphorylated by IKKα and IKKβ, leading to the degradation of IκB proteins and nuclear translocation of NF-κB proteins. Post-translation modification such as phosphorylation of NF-κB p65 at Thr435 site leads to transcription activity and NF-κB subunit transactivation. O’Shea and Perkins. Biochem J. 2010. 426: 345-354 Hayden and Ghosh. (2012) Genes and Dev 26: 203-234 Baeuerle and Henkel. (1994) Ann Rev Immunol 12: 141-179 Related news: Exploring Antiviral Immune Response
靶点名称	NFkB p65
別名	Phospho NFkB p65 antibody; NFkB p65 phospho (Thr435) antibody; NFkB p65 antibody

属性

存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

全名	Phospho NFkB p65 Antibody Duo (Total, pT435)
研究领域	Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Immune System antibody; Metabolism antibody; Microbiology and Infectious Disease antibody; Neuroscience antibody; Signaling Transduction antibody

检测图片 (6)

ARG51516 anti-NFkB p65 phospho (Thr435) antibody WB image
Western blot: MLE-12 stained with ARG51516 anti-NFkB p65 phospho (Thr435) antibody.
From Tian Li et al. Oxid Med Cell Longev. (2021), doi: 10.1155/2021/4158495, Fig. 3. a.
ARG65677 anti-NFkB p65 antibody WB image
Western blot: 30 µg of HeLa cell lysate stained with ARG65677 anti-NFkB p65 antibody at 1:500 dilution.
ARG51516 anti-NFkB p65 phospho (Thr435) antibody WB image
Western Blot: extracts from HeLa cells untreated or treated with TNFa+CA stained with anti-NFkB p65 (phospho Thr435) antibody ARG51516.
ARG51516 anti-NFkB p65 phospho (Thr435) antibody IHC-P image
Immunohistochemistry: paraffin-embedded human breast carcinoma tissue stained with anti-NFkB p65 (phospho Thr435) antibody ARG51516).
ARG51516 anti-NFkB p65 phospho (Thr435) antibody ICC/IF image
Immunofluorescence: methanol-fixed HeLa cells stained with anti-NFkB p65 (phospho Thr435) antibody ARG51516, Red).
ARG65677 anti-NFkB antibody IHC image
Immunohistochemistry: Mouse hippocampus tissue stained with ARG65677 anti-NFkB antibody at 1:200 dilution.