Phospho-PKA R2 (S99) Rabbit Monoclonal Antibody(ARA722)

Key features and details

Reactivity:Human, Mouse, Rat
Application:WB, IF, IHC, IP, FC
Host:Rabbit
Clonality:Monoclonal
lsotype: IgG
Target:Phospho-PKA R2 (S99)

Brand:

CAT.NO. : ARA6507

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Size:

50μL 100μL

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Product Details

Background

The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified, designated Cα, Cβ and Cγ, that each represent specific gene products. Cα and Cβ are closely related (93% amino acid sequence similarity), whereas Cγ displays 83% and 79% similarity to Cα and Cβ, respectively. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus where it phosphorylates the transcription factor cAMP response element binding protein (CREB) on serine 133 which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the pol II transcription initiation complex.

Application

Application	Dilution Ratio
WB	1:1,800 - 1:2,000
IF	1:50 - 1:200
IHC	1:800 - 1:1,000
IP	1-2μg/sample
FC	1:800 - 1:1,000

Overview

Antibody Type	Recombinant Rabbit monoclonal Antibody
Immunogen	Synthetic phospho-peptide corresponding to residues surrounding Ser99 of Human PKA R2 aa 82-126 / 404.
Species Reactivity	Human, Mouse, Rat
Validated Applications	WB, IF, IHC, IP, FC
Molecular Weight	Predicted band size: 46 kDa
Positive Control	PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate, human kidney tissue, mouse kidney tissue, rat kidney tissue, human colon cancer tissue, PC-12 cells treated with 100nM Calyculin A for 30 minutes, PC-12.
Conjugation	unconjugated
RRID	AB_3069912
Form	Liquid
Storage Buffer	1×TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype	IgG
Purification Method	Protein A affinity purified.

Data

Western blot analysis of Phospho-PKA R2 (S99) on different lysates with Phospho-PKA R2 (S99) Rabbit Monoclonal Antibody(ARA722).

Immunohistochemical analysis of paraffin-embedded rat kidney tissue untreated / treated with λpp with Phospho-PKA R2 (S99) Rabbit Monoclonal Antibody(ARA722).

Immunocytochemistry analysis of PC-12 cells untreated / treated with 100nM Calyculin A for 30 minutes labeling Phospho-PKA R2 (S99) with Phospho-PKA R2 (S99) Rabbit Monoclonal Antibody(ARA722).