Phosphorylated Neurofilament NF-H ELISA Kit
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CAT.NO. : ARG81164
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Product Details
概述
| 产品描述 | ARG81164 Phosphorylated Neurofilament NF-H ELISA Kit is an Enzyme Immunoassay kit for the quantification of Phosphorylated Neurofilament NF-H in plasma, serum, CSF and tissue extracts. |
|---|---|
| 反应物种 | Hu, Ms, Rat, Chk, Cow, Hrs, Pig |
| 应用 | ELISA |
| 靶点名称 | Neurofilament NF-H |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm |
| 样品类型 | Plasma, serum, CSF and tissue extracts. |
| 标准范围 | 0.156 - 10 ng/ml |
| 样本量 | 50 µl |
| 精确 | Intra-Assay CV: less than 6%; Inter-Assay CV: less than 10% |
| 別名 | Neurofilament heavy polypeptide; 200 kDa neurofilament protein; NF-H; Neurofilament triplet H protein; NFH |
应用说明
| 检测时间 | ~ 3 hours |
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属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store components at 4°C or -20°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | NEFH |
| 全名 | neurofilament, heavy polypeptide |
| 背景介绍 | Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. [provided by RefSeq, Oct 2008] |
| 生物功能 | Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins. [UniProt] |
| 产品亮点 | Related products: Neurofilament antibodies; Neurofilament ELISA Kits; Neurofilament Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 预测分子量 | 110 kDa |
| 翻译后修饰 | There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function. Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization. |
检测图片 (1)
ARG81164 Phosphorylated Neurofilament NF-H ELISA Kit standard curve image
ARG81164 Phosphorylated Neurofilament NF-H ELISA Kit results of a typical standard run with optical density reading at 450 nm.
参考文献
Therapeutic Effects of Combination of Nebivolol and Donepezil: Targeting Multifactorial Mechanisms in ALS
ELISA / Mouse / Serum
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