PIT-1/POU1F1 Rabbit Monoclonal Antibody(ARA967)
Key features and details
- Reactivity:Human
- Application:IHC-P
- Host:Rabbit
- Clonality:Monoclonal
- Target:PIT-1/POU1F1
-
Brand:
CAT.NO. : ARA6830
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Product Details
Background
PIT-1/POU1F1, known as growth hormone factor-1 (GHF-1), is a member of the POU homeodomain family, and essential for the normal development of the anterior pituitary gland, where it is required to form somatotropes, lactotropes, and thyrotropes.
Transcriptional regulators play a critical role in development by mediating tissue- and cell-specific transcription. POU domain factors are transcriptional regulators characterized by a bipartite DNA binding domain consisting of two highly conserved regions, tethered by a variable linker of 14-26 amino acids.
In somatotropes and lactotropes, PIT-1 activates the production of growth hormone and prolactin, respectively. In addition, PIT-1 acts as a repressor of gene expression, which allows for the differentiation of specific cell types. Pit-1 is expressed as two alternatively spliced products, designated PIT-1a and PIT-1b, which differ in their trans-activation ability.
Mutations in the PIT-1 gene are believed to result in combined pituitary hormone deficiency (CPHD) for growth hormone, Prolactin, and thyroid-stimulating hormone. The gene which encodes PIT-1 maps to human chromosome 3p11.2.
Transcriptional regulators play a critical role in development by mediating tissue- and cell-specific transcription. POU domain factors are transcriptional regulators characterized by a bipartite DNA binding domain consisting of two highly conserved regions, tethered by a variable linker of 14-26 amino acids.
In somatotropes and lactotropes, PIT-1 activates the production of growth hormone and prolactin, respectively. In addition, PIT-1 acts as a repressor of gene expression, which allows for the differentiation of specific cell types. Pit-1 is expressed as two alternatively spliced products, designated PIT-1a and PIT-1b, which differ in their trans-activation ability.
Mutations in the PIT-1 gene are believed to result in combined pituitary hormone deficiency (CPHD) for growth hormone, Prolactin, and thyroid-stimulating hormone. The gene which encodes PIT-1 maps to human chromosome 3p11.2.
Application
|
Application |
Dilution Ratio |
|
IHC-P |
1:100 - 1:200 |
Overview
|
Species Reactivity |
Humans; others not tested |
|
Known Applications |
Immunohistochemistry (formalin-fixed, paraffin-embedded tissues) |
|
Supplied As |
Buffer with protein carrier and preservative |
|
Control |
Pituitary |
|
Visualization |
Nucleus |
|
Isotype |
IgG |
|
Immunogen |
Recombinant human full-length POU1F1 protein |
Data
Immunohistochemical staining of human pituitary tissue sections using PIT-1/POU1F1 Rabbit Monoclonal Antibody (ARA967).
Storage
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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