pVHL Mouse Monoclonal Antibody(ARA966)
Key features and details
- Reactivity:Human
- Application:IHC-P
- Host:Mouse
- Clonality:Monoclonal
- Target:pVHL
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Brand:
CAT.NO. : ARA6829
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Product Details
Background
Von Hippel–Lindau syndrome (VHL) is a dominantly inherited hereditary cancer syndrome predisposing to a variety of malignant and benign tumors of the eye, brain, spinal cord, kidney, pancreas, and adrenal glands. A germline mutation of this gene is the basis of familial inheritance of VHL syndrome. Individuals with VHL syndrome inherit one mutation in the VHL protein that causes the protein's normal function to be lost or altered. Over time, sporadic mutation in the second copy of the VHL protein can lead to carcinomas, in particular hemangioblastomas affecting the liver and kidneys, renal (and vaginal) clear cell adenocarcinomas. The protein encoded by this gene is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This complex is involved in the ubiquitination and degradation of a hypoxia-inducible factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Application
|
Application |
Dilution Ratio |
|
IHC-P |
1:100 - 1:300 |
Overview
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Product Description |
Mouse monoclonal antibody to Von Hippel Lindau |
|
Immunogen |
KLH-conjugated synthetic peptide encompassing a sequence within human Von Hippel Lindau. The exact sequence is proprietary |
|
Purification Method |
The antibody was purified by immunogen affinity chromatography |
|
Clonality |
Monoclonal |
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Isotype |
Mouse IgG2b |
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Form |
Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide |
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Gene Name |
VHL |
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Synonyms |
Von Hippel-Lindau disease tumor suppressor; Protein G7; pVHL |
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Gene ID (Human) |
7428 |
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Protein ID (Human) |
P40337 |
Data
Immunohistochemical analysis of Von Hippel Lindau staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunohistochemical analysis of Von Hippel Lindau staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Storage
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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