Pyruvate Carboxylase Assay Kit
Key features and details
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CAT.NO. : ARG83429
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Product Details
概述
| 产品描述 | ARG83429 Pyruvate Carboxylase Assay Kit can be used to measure Pyruvate Carboxylase in urine, serum, plasma, tissue extracts, cell lysate, cell culture media and other biological fluids. |
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| 反应物种 | Other |
| 应用 | FuncSt |
| 靶点名称 | Pyruvate Carboxylase |
| 偶联标记说明 | Read at 340 nm |
| 灵敏度 | 0.001 μmol/ml |
| 样品类型 | Urine, serum, plasma, tissue extracts, cell lysate, cell culture media and other biological fluids. |
| 标准范围 | 0.004 μmol/ml - 0.4 μmol/ml |
| 样本量 | 10 μl |
| 別名 | Pyruvate carboxylase; PC; |
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 4°C, -20°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 背景介绍 | Pyruvate carboxylase was first discovered in 1959 at Case Western Reserve University by M. F. Utter and D. B. Keech. Since then it has been found in a wide variety of prokaryotes and eukaryotes including fungi, bacteria, plants, and animals. In mammals, PC plays a crucial role in gluconeogenesis and lipogenesis, in the biosynthesis of neurotransmitters, and in glucose-induced insulin secretion by pancreatic islets. Oxaloacetate produced by PC is an important intermediate, which is used in these biosynthetic pathways. In mammals, PC is expressed in a tissue-specific manner, with its activity found to be highest in the liver and kidney (gluconeogenic tissues), in adipose tissue and lactating mammary gland (lipogenic tissues), and in pancreatic islets. Activity is moderate in brain, heart and adrenal gland, and least in white blood cells and skin fibroblasts. |
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| 生物功能 | Pyruvate carboxylase (PC) encoded by the gene PC is an enzyme (EC 6.4.1.1) of the ligase class that catalyzes the physiologically irreversible[citation needed] carboxylation of pyruvate to form oxaloacetate (OAA).It is an important anaplerotic reaction that creates oxaloacetate from pyruvate. The enzyme is a mitochondrial protein containing a biotin prosthetic group, requiring magnesium or manganese and acetyl-CoA. |
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