Rabbit anti-NUPR1 Polyclonal Antibody

Key features and details

  • Reactivity: Human, Mouse, Monkey
  • Application: WB, IHC-P, IHC-F, IF, ELISA
  • Host: Rabbit
  • Clonality: Polyclonal
  • Isotype: IgG
  • Target Name: NUPR1
  • Brand:
CAT.NO. : ARA0001
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Product Details
Background
Could participate in the response to proapoptotic stimuli and promotes cellular growth in a way that helps the tissue counteract diverse injuries. May contribute to the metastatic phenotype.Tissue specificity: Highly expressed in pancreas.
Application

WB

1:500-2000

 IHC-P

1:100-500

 IHC-F

1:100-500

 IF

1:100-500

 ELISA

1:5000-10000

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Tissue specificity
Widely expressed, with high levels in liver, pancreas, prostate, ovary, colon, thyroid, spinal cord, trachea and adrenal gland, moderate levels in heart, placenta, lung, skeletal muscle, kidney, testis, small intestine, stomach and lymph node, and low levels in brain, spleen, thymus and bone marrow. Not detected in peripheral blood leukocytes.
Data

Paraformaldehyde-fixed, paraffin embedded (Mouse pancreas).
Antigen retrieval by boiling in sodium citrate buffer for 15 min.
Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes.
Blocking buffer at 37°C for 30 min.
Antibody incubation with (NUPR1) Polyclonal Antibody, Unconjugated secondary primary antibody at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.
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