Rat Activin A ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG82078
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82078-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82078-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82078-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82078-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82078-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82078-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82078-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82078-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82078-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82078-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82078-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82078 Rat Activin A ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat Activin A in serum, plasma (heparin, EDTA), saliva and cell culture supernatants. |
|---|---|
| 反应物种 | Rat |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | Activin A |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 7.8 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA), saliva and cell culture supernatants. |
| 标准范围 | 15.6 - 1000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.0%; Inter-Assay CV: 5.8% |
| 別名 | Erythroid differentiation protein; Activin beta-A chain; FRP; EDF; Inhibin beta A chain |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | INHBA |
| 全名 | inhibin, beta A |
| 背景介绍 | The inhibin beta A subunit joins the alpha subunit to form a pituitary FSH secretion inhibitor. Inhibin has been shown to regulate gonadal stromal cell proliferation negatively and to have tumor-suppressor activity. In addition, serum levels of inhibin have been shown to reflect the size of granulosa-cell tumors and can therefore be used as a marker for primary as well as recurrent disease. Because expression in gonadal and various extragonadal tissues may vary severalfold in a tissue-specific fashion, it is proposed that inhibin may be both a growth/differentiation factor and a hormone. Furthermore, the beta A subunit forms a homodimer, activin A, and also joins with a beta B subunit to form a heterodimer, activin AB, both of which stimulate FSH secretion. Finally, it has been shown that the beta A subunit mRNA is identical to the erythroid differentiation factor subunit mRNA and that only one gene for this mRNA exists in the human genome. [provided by RefSeq, Jul 2008] |
| 生物功能 | Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. [UniProt] |
| 细胞定位 | Secreted. [UniProt] |
| 产品亮点 | Related products: Activin A antibodies; Activin A ELISA Kits; Activin A recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1)
ARG82078 Rat Activin A ELISA Kit standard curve image
ARG82078 Rat Activin A ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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