Rat anti-Mouse IgA antibody [11-44-2]

Key features and details

  • 产品描述: Rat Monoclonal antibody [11-44-2] recognizes Mouse IgA
  • 反应物种: Ms
  • 应用: ELISA, FACS, FLISA, IHC-Fr, IHC-P, WB
  • 宿主: Rat
  • 克隆: Monoclonal
  • 克隆号: 11-44-2
  • 同位型: IgG1, kappa
  • 靶点名称: IgA
  • 抗原物种: Mouse
  • Brand:
CAT.NO. : ARG21616
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Product Details
概述
产品描述Rat Monoclonal antibody [11-44-2] recognizes Mouse IgA
反应物种Ms
应用ELISA, FACS, FLISA, IHC-Fr, IHC-P, WB
宿主Rat
克隆Monoclonal
克隆号11-44-2
同位型IgG1, kappa
靶点名称IgA
抗原物种Mouse
偶联标记Un-conjugated
应用说明
应用建议
应用推荐稀释比
ELISA1 - 0.05 μg/ml
FACS0.2 - 1 μg/10^6 cells
FLISAAssay-dependent
IHC-Fr2 - 5 μg/ml
IHC-P2 - 10 μg/ml
WBAssay-dependent
应用说明IHC-P: Antigen Retrieval: Boil tissue section in Citrate buffer (pH 6.0) for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
缓冲液BBS (pH 8.2)
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
检测图片 (3)
  • ARG21616 Rat anti-Mouse IgA antibody [11-44-2] IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse small intestine tissue. Antigen Retrieval: Boil tissue section in Citrate buffer (pH 6.0) for 20 min. The tissue section was stained with ARG21616 Rat anti-Mouse IgA antibody [11-44-2] (right) at 2 - 10 µg/ml dilution followed by an HRP-conjugated secondary antibody, DAB and hematoxylin. Control (left).

  • ARG21616 Rat anti-Mouse IgA antibody [11-44-2] ELISA image

    ELISA: The plate was coated with purified Mouse IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted ARG21616 Rat anti-Mouse IgA antibody [11-44-2] followed by ARG21696 Mouse anti-Rat IgG1 antibody [G17E7] (HRP).

  • ARG21616 Rat anti-Mouse IgA antibody [11-44-2] IHC-Fr image

    Immunohistochemistry: Frozen section of Mouse intestine tissue stained with ARG20689 Rat IgG1 Isotype Control antibody [KLH/G1-2-2] (left) and ARG21616 Rat anti-Mouse IgA antibody [11-44-2] (right) (2-5 μg/ml) followed by ARG21695 Mouse anti-Rat IgG1 antibody [G17E7] (FITC) and DAPI.

参考文献

Weissella paramesenteroides WpK4 ameliorate the experimental amoebic colitis by increasing the expression of MUC-2 and the intestinal epithelial regeneration.

IHC-P / Mouse

Prado GKS et al.
J Appl Microbiol.,  (2020)

publication_link

 

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