Rat Apolipoprotein B ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG82626
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Product Details
概述
| 产品描述 | ARG82626 Rat Apolipoprotein B ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat Apolipoprotein B in serum, plasma and cell culture supernatants. |
|---|---|
| 反应物种 | Rat |
| 应用 | ELISA |
| 特异性 | The following recombinant Rat proteins were tested and exhibited no cross-reactivity or interference: ApoAI, BMP1, BMP2, BMP3, BMP4, BMP7, CRP, HGF, HSP27, IL1 alpha, IL1 RI, IL1 beta, IL1RA, IL2, IL4, IL5, IL6, IL8, IL10, IL12, IL13, IL15, IL17C, IL21, IL23, IFN gamma, MMP2, sIL2R, sIL6R, PDGF, PLA2G7, prolactin, TGF beta 1, TGF beta 2, TGF beta 3, TLR1, TLR2, TLR3, TNF alpha, TNF RI, TNF RII and VEGF. |
| 靶点名称 | Apolipoprotein B |
| 偶联标记 | HRP |
| 偶联标记说明 | Read at 450 nm. |
| 灵敏度 | 6.3 ng/ml |
| 样品类型 | Serum, plasma and cell culture supernatants. |
| 标准范围 | 12.5 - 800 ng/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: less than 10% Inter-Assay CV: less than 10% |
| 別名 | Apolipoprotein B-100; apoB-48; Apo B-100; apoB-100; Apo B-48; FLDB; LDLCQ4 |
应用说明
| 检测时间 | ~ 3 hours |
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属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | APOB |
| 全名 | apolipoprotein B |
| 背景介绍 | This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq, Jul 2008] |
| 生物功能 | Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. [UniProt] |
| 细胞定位 | Cytoplasm. Secreted. [UniProt] |
| 产品亮点 | Related products: Apolipoprotein antibodies; Apolipoprotein ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Palmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle. [UniProt] |
检测图片 (1)
ARG82626 Rat Apolipoprotein B ELISA Kit standard curve image
ARG82626 Rat Apolipoprotein B ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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