Rat Endostatin ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG81910
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81910-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81910-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81910-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81910-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81910-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81910-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81910-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81910-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81910-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81910-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81910-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81910 Rat Endostatin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat Endostatin in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| 反应物种 | Rat |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | Endostatin |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 78 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| 标准范围 | 156 - 10000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.9%; Inter-Assay CV: 6.7% |
| 別名 | KS; KNO; KNO1; Collagen alpha-1(XVIII) chain |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | COL18A1 |
|---|---|
| 全名 | collagen, type XVIII, alpha 1 |
| 背景介绍 | This gene encodes the alpha chain of type XVIII collagen. This collagen is one of the multiplexins, extracellular matrix proteins that contain multiple triple-helix domains (collagenous domains) interrupted by non-collagenous domains. A long isoform of the protein has an N-terminal domain that is homologous to the extracellular part of frizzled receptors. Proteolytic processing at several endogenous cleavage sites in the C-terminal domain results in production of endostatin, a potent antiangiogenic protein that is able to inhibit angiogenesis and tumor growth. Mutations in this gene are associated with Knobloch syndrome. The main features of this syndrome involve retinal abnormalities, so type XVIII collagen may play an important role in retinal structure and in neural tube closure. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] |
| 生物功能 | COLA18A probably plays a major role in determining the retinal structure as well as in the closure of the neural tube. Endostatin potently inhibits endothelial cell proliferation and angiogenesis. May inhibit angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling. [UniProt] |
| 产品亮点 | Related products: Endostatin antibodies; Endostatin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Prolines at the third position of the tripeptide repeating unit (G-X-Y) of the triple-helical regions are hydroxylated. [UniProt] |
检测图片 (1)
ARG81910 Rat Endostatin ELISA Kit standard curve image
ARG81910 Rat Endostatin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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