Rat GDF5 ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
- 标准范围:
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Brand:
CAT.NO. : ARG82351
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82351-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82351-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82351-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82351-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82351-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82351-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82351-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82351-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82351-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82351-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82351-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82351 Rat GDF5 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat GDF5 in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| 反应物种 | Rat |
| 应用 | ELISA |
| 靶点名称 | GDF5 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 15.6 pg/ml |
| 样品类型 | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| 标准范围 | 31.2 - 2000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.5% Inter-Assay CV: 6.8% |
| 別名 | LAP-4; CDMP-1; Cartilage-derived morphogenetic protein 1; SYM1B; GDF-5; Radotermin; BDA1C; OS5; LAP4; Bone morphogenetic protein 14; BMP-14; BMP14; Lipopolysaccharide-associated protein 4; LPS-associated protein 4; SYNS2; Growth/differentiation factor 5; CDMP1 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | GDF5 |
|---|---|
| 全名 | growth differentiation factor 5 |
| 背景介绍 | The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Mutations in this gene are associated with acromesomelic dysplasia, Hunter-Thompson type; brachydactyly, type C; and chondrodysplasia, Grebe type. These associations confirm that the gene product plays a role in skeletal development. [provided by RefSeq, Jul 2008] |
| 生物功能 | Growth factor involved in bone and cartilage formation. During cartilage development regulates differentiation of chondrogenic tissue through two pathways. Firstly, positively regulates differentiation of chondrogenic tissue through its binding of high affinity with BMPR1B and of less affinity with BMPR1A, leading to induction of SMAD1-SMAD5-SMAD8 complex phosphorylation and then SMAD protein signaling transduction. Secondly, negatively regulates chondrogenic differentiation through its interaction with NOG. Required to prevent excessive muscle loss upon denervation. This function requires SMAD4 and is mediated by phosphorylated SMAD1/5/8 (By similarity). Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes. [UniProt] |
| 细胞定位 | Secreted. Cell membrane. [UniProt] |
| 产品亮点 | Related products: GDF5 ELISA Kits; GDF5 recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1)
ARG82351 Rat GDF5 ELISA Kit standard curve image
ARG82351 Rat GDF5 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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