Rat IGF1 ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
- 标准范围:
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Brand:
CAT.NO. : ARG82324
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82324-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82324-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82324-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82324-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82324-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82324-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82324-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82324-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82324-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82324-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82324-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82324 Rat IGF1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat IGF1 in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| 反应物种 | Rat |
| 应用 | ELISA |
| 靶点名称 | IGF1 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 31.25 pg/ml |
| 样品类型 | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| 标准范围 | 62.5 - 4000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.7% Inter-Assay CV: 6.7% |
| 別名 | MGF; Insulin-like growth factor I; Mechano growth factor; Somatomedin-C; IGFI; IGF-I |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | IGF1 |
| 全名 | insulin-like growth factor 1 (somatomedin C) |
| 背景介绍 | The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein. [provided by RefSeq, Sep 2015] |
| 生物功能 | The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. [UniProt] |
| 细胞定位 | Secreted. [UniProt] |
| 产品亮点 | Related products: IGF1 antibodies; IGF1 ELISA Kits; IGF1 Duos / Panels; IGF1 recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1)
ARG82324 Rat IGF1 ELISA Kit standard curve image
ARG82324 Rat IGF1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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