Rat TGFBI ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG81964
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81964-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81964-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81964-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81964-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81964-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81964-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81964-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81964-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81964-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81964-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81964-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81964 Rat TGFBI ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat TGFBI in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| 反应物种 | Rat |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | TGFBI |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 78 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| 标准范围 | 156 - 10000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.9%; Inter-Assay CV: 6.6% |
| 別名 | CDGG1; LCD1; RGD-CAP; CSD2; CSD; Beta ig-h3; CSD1; Transforming growth factor-beta-induced protein ig-h3; RGD-containing collagen-associated protein; BIGH3; CDG2; CSD3; Kerato-epithelin; CDB1; EBMD |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | TGFBI |
|---|---|
| 全名 | transforming growth factor, beta-induced, 68kDa |
| 背景介绍 | This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion. Mutations in this gene are associated with multiple types of corneal dystrophy. [provided by RefSeq, Jul 2008] |
| 生物功能 | Binds to type I, II, and IV collagens. This adhesion protein may play an important role in cell-collagen interactions. In cartilage, may be involved in endochondral bone formation. [UniProt] |
| 产品亮点 | Related products: TGFBI antibodies; TGFBI ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Gamma-carboxylation is controversial. Gamma-carboxyglutamated; gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation; these residues may be required for binding to calcium (PubMed:18450759). According to a more recent report, does not contain vitamin K-dependent gamma-carboxyglutamate residues (PubMed:26273833). The EMI domain contains 2 expected intradomain disulfide bridges (Cys-49-Cys85 and Cys-84-Cys-97) and one unusual interdomain disulfide bridge to the second FAS1 domain (Cys-74-Cys-339). This arrangement violates the predicted disulfide bridge pattern of an EMI domain. [UniProt] |
检测图片 (1)
ARG81964 Rat TGFBI ELISA Kit standard curve image
ARG81964 Rat TGFBI ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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