Rat VEGF ELISA Kit
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
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Brand:
CAT.NO. : AER0008
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Product Details
Background
Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues in a cystine knot structure and the formation of antiparallel disulfide-linked dimers. Humans express alternately spliced isoforms of 121, 145, 165, 183, 189, and 206 amino acids (aa) in length. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189. Isoforms other than VEGF121 contain basic heparin-binding regions and are not freely diffusible. Human VEGF165 shares 88% aa sequence identity with corresponding regions of mouse and rat VEGF. VEGF is expressed in multiple cells and tissues including skeletal and cardiac muscle, hepatocytes, osteoblasts, neutrophils, macrophages, keratinocytes, brown adipose tissue, CD34+ stem cells, endothelial cells, fibroblasts, and vascular smooth muscle cells. VEGF expression is induced by hypoxia and cytokines such as IL-1
TYPICAL DATA
| pg/ml | O.D. | Average | Corrected | |
| 0 | 0.0344 | 0.0324 | 0.0334 | |
| 6.86 | 0.0414 | 0.0409 | 0.0412 | 0.0078 |
| 20.58 | 0.0523 | 0.0536 | 0.053 | 0.0196 |
| 61.73 | 0.0835 | 0.0842 | 0.0839 | 0.0505 |
| 185.19 | 0.1739 | 0.1849 | 0.1794 | 0.146 |
| 555.56 | 0.5003 | 0.5008 | 0.5006 | 0.4672 |
| 1666.67 | 1.347 | 1.41 | 1.3785 | 1.3451 |
| 5000 | 3.612 | 3.725 | 3.6685 | 3.6351 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 116.6 | 544.5 | 1494.4 | 116.7 | 528.9 | 1673.6 |
| Standard Deviation | 8.4 | 23.5 | 93.1 | 5.7 | 31.8 | 74.6 |
| Coefficient of Variation(%) | 7.2 | 4.3 | 6.2 | 4.9 | 6 | 5.2 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of rat VEGF into health rat serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 89% to 151% with an overall mean recovery of 112%.
The recovery ranged from 89% to 151% with an overall mean recovery of 112%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
| Serum | 30 | 4.09-116.90 | 50 | 22.78 |
Serum/Plasma – Thirty samples from apparently healthy rats were evaluated for the presence of VEGF in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
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