Recombinant Cys-Protein G

Key features and details

  • Species:
  • Abbreviation: Cys-Protein G
  • Source: Expressed in E. coli.
  • Molecular Weight: Approximately 21.9 kDa, and it migrates with an apparent molecular mass of 40 kDa in SDS-PAGE.
  • Purity: >95% by SDS-PAGE or HPLC.
  • Endotoxin: < 0.1 EU/μg of Cys-Protein G protein as determined by LAL method.
  • Accession #: P19909 Leu298-Glu495 with an N-terminal Cys
  • Storage:
  • Brand:
CAT.NO. : ARP6781
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Product Details
Background
Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcic. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but can also bind to the Fab region, making it useful for purification of F (ab') fragments of IgG. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology Recombinant Cys-Protein G is a single non-glycosylated polypeptide chain containing  201 amino acids. and there is an N-terminal cysteine for Site-Specific Conjugation.
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