Recombinant Enhanced Green Fluorescent Protein
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CAT.NO. : ARP6631
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Product Details
Background
The recombinant eGFP was expressed and purified from transformed E. coli using a method that ensures high purity and maximal eGFP fluorescence. Endotoxin was removed, so the protein is suitable for in vivo injection or cell culture applications. Green fluorescent protein (GFP) here refers to the protein first purified from jellyfish Aequorea Victoria, though many other organisms have similar proteins. It is a 26.9kDa protein (composed of 238 AA residues) that shows green fluorescence in short-wave light (blue to ultraviolet) . Enhanced GFP (eGFP) has S65T and F64L mutations, which make GFP show increased fluorescence and fold more efficiently under 37℃, respectively. eGFP allows the use of GFP in mammalian cells. In A. Victoria, GFP plays roles as an energy transfer acceptor. It has long been used in cell and molecular biology as a reporter of gene expression. GFP can also been applied as a molecular thermometer to measure temperature accurately in fluids.
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