Recombinant Human NOS2

Key features and details

  • Species: Human
  • Uniprot ID: P35228
  • Expression Region: 19-221
  • Host: E.Coli
  • Tags: N-terminal His Tag
  • Brand:
CAT.NO. : AXP0001
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Product Details
Description

Species

Human

Uniprot ID

P35228

Expression Region

19 - 221

Host

E.Coli

Tags

N - terminal His Tag

Molecular Weight

24.5 kDa under reducing conditions

Purity

Greater than 90% as determined by SDS - PAGE

Formulation

Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4

Reconstitution

Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1 - 1mg/ml by gently pipetting 2 - 3 times, don't vortex

Storage

The lyophilized protein is stable at - 20°C for up to 1 year, the protein solution can be stored at 2 - 8°C for up to 1 week under sterile condition.
For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment) and store in working aliquots at - 20°C to - 80°C for 6 months.
Avoid repeated freeze/thaw cycles

Synonyms
HEP-NOS, Hepatocyte NOS, HEPNOS, inducible, Inducible nitric oxide synthase, Inducible NO synthase, Inducible NOS, iNOS, MAC NOS, Macrophage NOS, Nitric oxide synthase, Nitric oxide synthase 2 inducible, Nitric oxide synthase 2 inducible macrophage
SDS-PAGE
Safety Note
This product is only for research and manufacturing purposes, not for diagnosis. Multiple freeze/thaw cycles can cause product degradation. It is recommended to store the antigen in aliquots of appropriate use - size and thaw them right before use. Although this material has been inactivated, like all biological materials, it should be treated as potentially infectious. The user is responsible for the care, custody, control, and disposal of the material in compliance with all relevant regulations.
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