RIPK1 Rabbit Monoclonal Antibody(YJY-5-8)
Key features and details
- Reactivity:Human
- Application:WB, IHC
- Host:Rabbit
- Clonality:Monoclonal
- Target:RIPK1
-
Brand:
CAT.NO. : ARA6604
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Size:
Trail, Bulk size or Custom requests Please contact us
Product Details
Background
Serine-threonine kinase which is a key regulator of TNF-mediated apoptosis, necroptosis and inflammatory pathways. Exhibits kinase activity-dependent functions that regulate cell death and kinase-independent scaffold functions regulating inflammatory signaling and cell survival. Has kinase-independent scaffold functions: upon binding of TNF to TNFR1, RIPK1 is recruited to the TNF-R1 signaling complex (TNF-RSC also known as complex I) where it acts as a scaffold protein promoting cell survival, in part, by activating the canonical NF-kappa-B pathway.
Application
|
Application |
Dilution Ratio |
|
WB |
1:500 - 1:2000 |
|
IHC |
1:10 - 1:100 |
Overview
|
Predicted Molecular Weight |
75kDa |
|
Species Cross-reactivity |
Human (Species cross-reactivity determined by WB) |
|
Purity |
ProA affinity purified IgG |
|
Form |
Liquid |
|
Swissprot ID |
Q13546 |
|
Immunogen |
A synthetic peptide corresponding to residues aa200-300 of human RIPK1 was used as an immunogen. |
|
Storage Buffer |
PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA |
Data
All lanes: RIPK1 Rabbit Monoclonal Antibody(YJY-5-8) at 1:2000 dilution
Predicted MW: 75 kDa
Observed MW: 70 kDa
Lane 1: WT Jurkat cell lysates
Lane 2: RIPK1 deficient Jurkat cell lysates
Lysates at approx. 10 μg per lane
2nd Ab: GAR HRP(H+L) 1:4,000
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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