SDHA Rabbit Monoclonal Antibody(ARA830)

Datasheet

Key features and details

Reactivity:Human
Application:WB,IHC-P,ICC/IF,IP
Host:Rabbit
Clonality:Monoclonal
Target:SDHA

Brand:

CAT.NO. : ARA6641

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Size:

100μL

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Product Details

Background

This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29.

Overview

Cross - Reactivity	Human, Mouse, Rat
Antibody Source	Rabbit
Immunogen	A synthesized peptide derived from human SDHA: 575 - 621
Isotype	IgG
Physical State	Liquid
Purification Method	affinity purified by Protein A
Clonality	Recombinant
Theoretical Molecular Weight	73
Detected Molecular Weight	72
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
SWISS	P31040
Gene ID	6389

Application

WB	1:500-2000
IHC-P	1:50-200
IHC-F	1:50-200
IF	1:50-200
Flow-Cyt	1:20-100
ICC/IF	1:50-200
IP	1:20-50

Data

25 ug total protein per lane of various lysates (see on figure) probed with SDHA monoclonal antibody, unconjugated at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.