Slug Mouse Monoclonal Antibody(ARA892)

Key features and details

Reactivity:Human, Mouse, Rat
Application:IHC
Host:Mouse
Clonality:Monoclonal
Target:Slug

Brand:

CAT.NO. : ARA6719

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Size:

1mL

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Product Details

Background

The SNAIL family of developmental regulatory proteins is a group of widely conserved zinc-finger proteins that regulate transcription and include the mammalian proteins SLUG, SNAI 1 (the human homolog of Drosophila SNAIL) and Smuc. SNAI 1 and SLUG are expressed in placenta and in adult heart, liver, and skeletal muscle. SNAI 1, and the corresponding mouse homolog Sna, each contain three classic zinc fingers and one atypical zinc finger, while SLUG contains five zinc finger regions and a transcriptional repression domain at the amino terminus, which enables SLUG to act as a negative regulator of gene expression. SLUG is implicated in the generation and migration of neural crest cells in human embryos and also contributes to limb bud development. In addition, SLUG also constitutes a cellular anti-apoptotic transcription factor that effectively prevents apoptosis in murine pro-B cells deprived of IL-3. The SNAIL-related gene from murine skeletal muscle cells, Smuc, is highly expressed in skeletal muscle and thymus and can, likewise, repress gene transcription. Smuc preferentially associates with CAGGTG and CACCTG E-box motifs (CANNTG) on DNA and involves the five putative DNA-binding zinc finger domains at the C-terminal region of Smuc.ZX

Application

Application	Dilution Ratio
IHC-P	1:200-1:1000

Overview

Antibody Type	Mouse Monoclonal Antibody
Immunogen	Purified recombinant fragment of human SNAI2 (AA: 100-200) expressed in E. Coli
Species Reactivity	Human
Validated Applications	WB, IF-Cell, IHC-P, FC
Molecular Weight	30 kDa
Positive Control	Human SNAI2 recombinant protein, SNAI2-HisGFc-transfected HEK293 cell lysate, HeLa, MCF-7, human colon cancer tissue, human cervical cancer tissue
Conjugation	unconjugated
RRID	AB_3070586
Form	Liquid
Concentration	2μg/ul
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide
Isotype	IgG1
Purification Method	Protein A affinity purified