SNAIL Rabbit Polyclonal Antibody
Key features and details
- Reactivity:Human, Mouse, Rat
- Application:WB, ICC/IF
- Host:Rabbit
- Clonality:polyclonal
- Target:SNAIL
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Brand:
CAT.NO. : ARA6484
RMB Please choose
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Product Details
Background
Zinc finger protein SNAI1 (sometimes referred to as Snail) is a protein that in humans is encoded by the SNAI1 gene. Snail is a family of transcription factors that promote the repression of the adhesion molecule E-cadherin to regulate epithelial to mesenchymal transition (EMT) during embryonic development. The Drosophila embryonic protein SNAI1, commonly known as Snail, is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila Snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2. SNAI1 zinc-fingers (ZF) binds to E-box, an E-cadherin promoter region, and represses the expression of the adhesion molecule, which induces the tightly bound epithelial cells to break loose from each other and migrate into the developing embryo to become mesenchymal cells. This process allows for the formation of the mesodermal layer in the developing embryo. Though SNAI1 is shown to repress expression of E-cadherin in epithelial cells, studies have shown homozygous mutant embryos are still able to form a mesodermal layer. However, the mesodermal layer present shows characteristics of epithelial cells and not mesenchymal cells (the mutant mesoderm cells exhibited a polarized state). Other studies show that mutation of specific ZFs contribute to a decrease in SNAI1 E-cadherin repression.
Application
|
Application |
Dilution Ratio |
|
WB |
1:500 - 1:5000 |
|
ICC/IF |
1:20 - 1:50 |
Overview
|
Product Name |
SNAIL Rabbit Polyclonal Antibody |
|
Antibody Type |
Rabbit Polyclonal Antibody |
|
Immunogen |
Recombinant protein within human SNAIL aa 1-200. |
|
Species Reactivity |
Human, Mouse, Rat |
|
Molecular Weight |
Predicted band size: 29 kDa |
|
Positive Control |
A549 cell lysate, 293T cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C6 cell lysate, NIH/3T3. |
|
Conjugation |
unconjugated |
|
RRID |
AB_3069038 |
|
Form |
Liquid |
|
Storage Buffer |
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
|
Isotype |
IgG |
|
Purification Method |
Immunogen affinity purified. |
Data
Western blot analysis of SNAIL on different lysates with SNAIL Rabbit Polyclonal Antibody.
Immunocytochemistry analysis of NIH/3T3 cells labeling SNAIL with SNAIL Rabbit Polyclonal Antibody.
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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