Tau (S305) Mouse Monoclonal Antibody(ARA983)

Tau (S305) refers to a specific site on the tau protein, where a mutation in the S305N form is associated with a form of frontotemporal dementia. Research shows that phosphorylation at the S305 site normally inhibits tau aggregation, a process linked to neurodegenerative diseases like Alzheimer's and frontotemporal dementia. Mutations at this site, such as S305N, increase the proportion of a specific tau isoform (4R) and can disrupt normal brain cell function.

Application	Dilution Ratio
WB	1:800 - 1:1000
Sandwich ELISA	1:250 - 1:500
IP	1:80 - 1:100

Antibody Specificity	Recognizing Tau protein containing S305 and S356 peptides that share a sequence of HVPGGG
Species Reactivity	Human, Mouse
Clonality	Monoclonal
Host / Isotype	Mouse / IgG2a
Immunogen	Recombinant human Tau-F
Cross Reactivity	Not tested
Recombinant	Not recombinant
Purification	Protein A purification
Form	Liquid
Conjugation	Unconjugated

Western blotting analysis of Tau (S305) by ARA6837.
15 µg of mouse brain tissue lysate was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. ARA6837 was used as the primary antibody and peroxidase conjugated anti-mouse IgG (Light chain specific) was used as the secondary antibody. Tau band was visualized using ECL Western Blotting Substrate.
Result: ARA6837 can detect Tau by Western blotting.


Immunoprecipitation analysis of Tau (S305) by ARA6837.
Immunoprecipitation was performed by incubation of 2.5 µg of ARA6837 with brain tissue lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. Tau (RC-4594) was used as the primary antibody and peroxidase conjugated goat anti-rabbit IgG was used as the secondary antibody. 
Lane 1: Brain tissue lysate
Lane 2: Tau immunoprecipitated from brain tissue lysate by ARA6837
Lane 3: The same as Lane 2 but isotype control antibody was used as IgG isotype control antibody.
Result: ARA6837 can immunoprecipitate Tau.


Sandwich ELISA analysis of Tau (S305) by ARA6860 & ARA6807.
Microtiter wells were coated with ARA6807 as the capture antibody.
Recombinant TauProtein (Cat. PP-11309) was used as the antigen.
Peroxidase conjugated Tau Antibody (ARA6860) was used as the detection antibody.
Result: ARA6860 and ARA6807 can be used as a matched antibody pair to detect and quantify the concentration of Tau.


Cross reactivity of ARA6860 to other peptides and recombinant proteins by ELISA.
Microtiter wells were coated with various peptides and recombinant proteins.
ARA6860 was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody.
Result: ARA6860 does cross-react with human MAPT.


Epitope analysis of Tau (S305) by ARA6860 by ELISA.
Synthetic peptides containing indicated residues were coated onto microtiter wells. Recombinant Tau (2-244), Tau (1-368), Tau (243-368) and Tau (1-441) proteins with isoform P10636-8 or Tau (1-758) (with isoform P10636-1) were also coated. The antibody ARA6860 was applied as the primary antibody.
Peroxidase-conjugated goat anti-mouse IgG was used as the secondary antibody, and signals were detected using a colorimetric assay.
Result: ARA6860 recognizes Tau protein containing S305 and S356 peptides that share a sequence of HVPGGG.

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

For Research Use Only. Not for use in diagnostic procedures.