TFIIIC110 Mouse Monoclonal Antibody (2395C2a)
Key features and details
- Reactivity:Human
- Application:WB, IP
- Host:Mouse
- Clonality:Monoclonal
- lsotype:IgG1
- Target Name:TFIIC110
-
Brand:
CAT.NO. : ARA1123
US$ Please choose
US$ Please choose
Size:
Trail, Bulk size or Custom requests Please contact us
*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。
Product Details
Background
RNA polymerase (pol) III synthesizes tRNA, 5s rRNA, 7SL RNA and U6 snRNA and is overexpressed in many transformed cell lines and tumors in vivo, since cells must duplicate its protein components before division. Therefore, in order to maintain rapid growth, cells must produce a high level of Pol III transcribed RNA, which requires the presence of the TFIIIB and TFIIIC2 transcription factor complexes. The TFIIIC2 complex is composed of five subunits, TFIIIC220, TFIIIC110, TFIIIC102, TFIIIC90 and TFIIIC63, that are overexpressed in adenovirus transformed cells as well as in malignant cells in vivo, such as ovarian carcinomas. TFIIIC2 recruits RNA pol III and TFIIIB to promoter elements and may be a key component in the deregulation of malignant cells. The TFIIIB complex includes the TATA - binding protein (TBP), TFIIB - related factor 1 (BRF1) and TFIIIB″, the expression of which are also upregulated in transformed cells. In many carcinomas, the tumor suppressors retinoblastoma (RB) and p53 are inactivated, which affects their ability to bind and inactivate the function of TFIIIB.
Application
TFIIIC110 (2395C2a) is recommended for detection of TFIIIC110 of human origin by Western Blotting (starting dilution 1:200, dilution range 1:100 - 1:1000) and immunoprecipitation [1 - 2 µg per 100 - 500 µg of total protein (1 ml of cell lysate)].
Data
TFIIIC110 (2395C2a). Western Blot analysis of TFIIIC110 expression in HeLa whole cell lysate.
TFIIIC110 (2395C2a). Western Blot analysis of human recombinant TFIIIC110 fusion protein.
New Products
