TRA-1-60 Mouse Monoclonal Antibody (TRA-1-60)

Key features and details

Reactivity:Human
Application: WB, IP, IF, FCM
Host:Mouse
Clonality: Monoclonal
lsotype:IgM k
Target:TRA-1-60

Brand:

CAT.NO. : ARA0160

US$ Please choose

Size:

200μg

Trail, Bulk size or Custom requests Please contact us

*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。

Product Details

Background

Embryonic stem cells have the ability to remain undifferentiated and proliferate indefinitely in vitro, while maintaining the potential to differentiate into derivatives of all three embryonic germ layers. Undifferentiated human embryonal carcinoma (EC) cells are the stem cells of teratocarcinomas and are characterized by the expression of Stage Specific Embryonic Antigens SSEA - 1 and SSEA - 3, the high molecular weight glycoproteins TRA - 1 - 60 and TRA - 1 - 81, as well as TRA - 2 - 39 and TRA - 2 - 54. Monoclonal antibodies TRA - 2 - 49 and TRA - 2 - 54 also recognize the liver isozyme of alkaline phosphatase expressed by human EC cells. TRA - 1 - 60 antigen was originally identified as a teratocarcinoma mucin - like antigen expressed on the surface of EC progenitor cells. TRA - 1 - 60 is also characterized as a tumor marker for embryonal carcinoma positive NSTGCT (nonseminomatous testicular germ cell tumors) and is co - expressed with TRA - 1 - 81 and the SSEAs on the membrane of a considerable number of stem cells.

Application

TRA - 1 - 60 (TRA - 1 - 60) is recommended for detection of TRA - 1 - 60 of human origin by Western Blotting (starting dilution 1:200, dilution range 1:100 - 1:1000), immunoprecipitation [1 - 2 µg per 100 - 500 µg of total protein (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution range 1:50 - 1:500) and flow cytometry (1 µg per 1 x 10⁶ cells).

Data

Western blot analysis of TRA - 1 - 81 (A) and TRA - 1 - 60 (B) expression in NTERA - 2 cl.D1 whole cell lysate. Antibodies tested include TRA - 1 - 81 (TRA - 1 - 80(A) and TRA - 1 - 60 (TRA - 1 - 60)(B).