TRITC Conjugated Mouse IgG H&L Goat Polyclonal Antibody
Key features and details
- Reactivity:Mouse
- Application:IF/ICC, FC
- Host:Goat
- Clonality:Polyclonal
- Target:Mouse IgG H&L
-
Brand:
CAT.NO. : ARA6499
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Size:
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Product Details
Background
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.TRITC(tetraethyl rhodamine isothiocyanate) is the form of fluorescein used for conjugation to all of our antibodies and purified proteins, with the exception of streptavidin.The maximum absorption light wavelength is 550nm, and the maximum emission light wavelength is 620nm, showing orange-red fluorescence. Compared with FITC's emerald green fluorescence, it can be used for double labeling or contrast staining.
Application
|
Application |
Dilution Ratio |
|
IF/ICC |
1:50 - 1:500 |
|
FC |
1:50 - 1:200 |
Overview
|
Antibody Type |
Goat Polyclonal Antibody |
|
Immunogen |
Mouse IgG (H+L) |
|
Product Specificity |
To minimize cross-reactivity, the TRITC conjugated goat anti-mouse IgG (H+L) antibodies have been highly cross-adsorbed against human IgG. |
|
Species Reactivity |
Mouse |
|
Validated Applications |
IF/ICC, FC |
|
Form |
Liquid |
|
Storage Buffer |
1×TBS (pH7.4), 0.5%BSA, 40%Glycerol. |
|
Isotype |
IgG |
|
Purification Method |
Immunogen affinity purified. |
Data
ICC staining Vimentin(1/200) in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. TRITC Conjugated Mouse IgG H&L Goat Polyclonal Antibody (1/100)as used as the secondary antibody.
Results of IF-Tissue assay on paraffin-embedded sections of human kidney tissue, with Vimentin Mouse Monoclonal Antibody used as the primary antibody and TRITC Conjugated Mouse IgG H&L Goat Polyclonal Antibody as the secondary antibody.
Flow cytometric analysis of Hela cells with Vimentin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is TRITC Conjugated Mouse IgG H&L Goat Polyclonal Antibody at 1/100 dilution.
Storage
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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