Troponin T Rabbit Monoclonal Antibody(ARA913)

Key features and details

  • Reactivity:Human, Mouse, Rat
  • Application:IHC
  • Host:Rabbit
  • Clonality:Monoclonal
  • Target:Troponin T
  • Brand:
CAT.NO. : ARA6748
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Size:
1mL
Trail, Bulk size or Custom requests Please contact us
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Product Details
Background
Actin is a highly conserved protein that is expressed in all eukaryotic cells. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. Myosin is a hexamer of two heavy chains (MHC) and four light chains (MLC) that interacts with Actin to generate the force for diverse cellular movements, including cytokinesis, phagocytosis and muscle contraction. Troponin facilitates the interaction between Actin and Myosin by binding to calcium. Troponin is made up of at least two subunits, which are divergent in cardiac muscle, fast skeletal muscle and slow skeletal muscle. Structures of skeletal muscle troponin are composed of Troponin C (the sensor), Troponin I (the regulator) and Troponin T (the link to the muscle thin filament). Troponin C is dumbbell-shaped and has a hydrophobic pocket that increases the contractile force of muscle fibers. Troponin C has two isoforms: fast and slow. Fast Troponin C has two calcium binding sites, while slow/cardiac Troponin C has a single calcium binding site.
Application

Application

Dilution Ratio

IHC-P

1:500-1:2000

Overview

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human Cardiac Troponin T aa 251-298 / 298

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IF-Tissue

Molecular Weight

Predicted band size: 36 kDa

Positive Control

Mouse heart tissue lysate, Rat heart tissue lysate, human heart tissue, mouse heart tissue, rat heart tissue

Conjugation

unconjugated

Form

Liquid

Concentration

1μg/μl

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide

Isotype

IgG

Purification Method

Protein A affinity purified

Data

Immunohistochemical staining of human heart tissue using Troponin T Rabbit Monoclonal Antibody(ARA913)
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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