Type II Cytokeratins Rabbit Monoclonal Antibody(ARB858)
Key features and details
- Target:
- Clone ID:
- Host:
- Molecular Weight:
- Purity:
- Species Cross-reactivity:
- Applications:
- Swissprot ID:
-
Brand:
CAT.NO. : ARB6649
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Size:
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Product Details
Background
The keratins are the typical intermediate filament proteins of epithelia, showing an outstanding degree of molecular diversity. Heteropolymeric filaments are formed by pairing of type I and type II molecules. In humans 54 functional keratin genes exist. They are expressed in highly specific patterns related to the epithelial type and stage of cellular differentiation.This antibody can detect high molecular weight CK1, CK2, CK3, CK4, CK5, CK6, CK7 and CK8 and . It is usually used in a cytokeratin cocktail with type I cytokeratins antibody.
Application
|
Application |
Dilution Ratio |
|
IHC |
1:100 - 1:200 |
Overview
| Target | Type II Cytokeratins |
| Host Species | rabbit monoclonal antibody |
| Molecular Weight | 66-54 kDa |
| Purity | ProA affinity purified IgG |
| Species Cross-reactivity | Human |
| Form | Liquid |
| Applications | IHC-P |
| Swissprot ID | Not available |
| Immunogen | Synthetic peptide corresponding to Type II Cytokeratins residues within aa300-400 |
| Storage Buffer | PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05% |
| Storage Conditions | -25°C to -18°C |
| Dilutions | IHC-P: 1:100-1:200 |
| Subcellular Location | Cytoplasm |
| Recommended Method | Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes |
Data
Immunohistochemical staining of human cervical carcinoma tissue sections using Type II Cytokeratins Rabbit Monoclonal Antibody (ARB858).
Immunohistochemical staining of human uterine tissue sections using Type II Cytokeratins Rabbit Monoclonal Antibody (ARB858).
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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