Vimentin Rabbit Monoclonal Antibody(ARB570)
Key features and details
- Target:
- Clone ID:
- Host:
- Molecular Weight:
- Purity:
- Species Cross-reactivity:
- Applications:
- Swissprot ID:
-
Brand:
CAT.NO. : ARB6862
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Size:
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Product Details
Background
Vimentin is the most common member of intermediate filament (IF) family and one of the main components in cytoskeleton structure. It is essential in the role of cell integrity and cytoskeletal stability. The reorganization of vimentin, similar to all IF proteins, occurs during different stages of the cell cycle and cell signaling by a site - specific phosphorylation (serine and threonine residues).
Vimentin is expressed in a wide variety of mesenchymal cell types: fibroblasts, endothelial cells etc., and in a number of other cell types derived from mesoderm, e.g., mesothelium and ovarian granulosa cells. However, in non - vascular smooth muscle cells, vimentin is often replaced by desmin. In striated muscle, vimentin is also replaced by desmin. However, during regeneration, vimentin is reexpressed. Cells of the lympho - hematopoietic system (lymphocytes, macrophages etc.) also express vimentin, sometimes in scarce amounts. In tumor tissues, it is present in many different neoplasms but is particulary expressed in those originated from mesenchymal cells.
In combination with a panel of antibodies, it is used to identify tumor with mesenchymal origin and malignant melanoma. Additionally, vimentin is a useful control marker for proper tissue processing.
Vimentin is expressed in a wide variety of mesenchymal cell types: fibroblasts, endothelial cells etc., and in a number of other cell types derived from mesoderm, e.g., mesothelium and ovarian granulosa cells. However, in non - vascular smooth muscle cells, vimentin is often replaced by desmin. In striated muscle, vimentin is also replaced by desmin. However, during regeneration, vimentin is reexpressed. Cells of the lympho - hematopoietic system (lymphocytes, macrophages etc.) also express vimentin, sometimes in scarce amounts. In tumor tissues, it is present in many different neoplasms but is particulary expressed in those originated from mesenchymal cells.
In combination with a panel of antibodies, it is used to identify tumor with mesenchymal origin and malignant melanoma. Additionally, vimentin is a useful control marker for proper tissue processing.
Application
|
Application |
Dilution Ratio |
|
IHC |
1:100 - 1:200 |
Overview
|
Predicted Molecular Wt |
54kDa |
|
Species Cross-reactivity |
Human |
|
Applications |
IHC-P |
|
Purity |
ProA affinity purified IgG |
|
Form |
Liquid |
|
Swissprot ID |
P08670 |
|
Subcellular location |
Cytoplasm |
|
Recommended method |
Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18℃-25℃) for 30 minutes |
|
Immunogen |
Synthetic peptide corresponding to Vimentin residues within aa366 - 466 of Vimentin was used as an immunogen |
|
Storage Buffer |
PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05% |
Data
Immunohistochemical staining of human breast cancer tissue using Vimentin Rabbit Monoclonal Antibody(ARB570)
Storage
Store at -20°C. Stable for one year from the date of shipment.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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