Vitamin H (Biotin) ELISA Kit

Key features and details

  • 产品描述: ARG80817 Vitamin H (Biotin) ELISA Kit is a Enzyme Immunoassay kit for the quantification of Vitamin H (Biotin) in food (extraction, dilution).
  • 反应物种: Other
  • 应用: ELISA
  • 靶点名称: Vitamin H
  • 偶联标记: HRP
  • 偶联标记说明: Substrate: TMB and read at 450 nm
  • 灵敏度: 0.5 ng/ml
  • 样品类型: Food (extraction, dilution).
  • 标准范围: 1 - 25 ng/ml
  • Brand:
CAT.NO. : ARG80817
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Product Details
概述
产品描述ARG80817 Vitamin H (Biotin) ELISA Kit is a Enzyme Immunoassay kit for the quantification of Vitamin H (Biotin) in food (extraction, dilution).
反应物种Other
应用ELISA
靶点名称Vitamin H
偶联标记HRP
偶联标记说明Substrate: TMB and read at 450 nm
灵敏度0.5 ng/ml
样品类型Food (extraction, dilution).
标准范围1 - 25 ng/ml
样本量50 µl
应用说明
检测时间1 h (RT/shaker), 20 min (RT/dark)
属性
形式96 well
存放说明Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
全名Vitamin H / Biotin
背景介绍Biotin serves as the prosthetic group of enzymes, which catalyze carboxylations in the organism. For this purpose, biotin is bound via its carboxy group to lysin residues of carboxylases, and the transfer of carbon dioxide takes place after its attachment to a nitrogen atom of biotin, forming the so-called active carbon dioxide.

The awareness of the population for a good health and its interest in healthy nutrition has increased sig­nificantly during the last years. After the content of vitamins in his nourishment has gained importance for the consumer, food has partially been vitaminized by the manufacturer.

When there exists a lack of biotin, seborrhoea, dermatitis, anorexia, muscle pain, tiredness and nervous disorders can appear. As biotin is synthesized by the human intestinal flora, deficiency symptoms are rare, appear however after excessive ingestion of raw egg white, which can be explained by its content of biotin-binding avidin.
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检测图片 (1)
  • ARG80817 Vitamin H (Biotin) ELISA Kit standard curve image

    ARG80817 Vitamin H (Biotin) ELISA Kit results of a typical standard run with optical density reading at 450nm.

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